RRC ID 53949
Author Fassnacht C, Tocchini C, Kumari P, Gaidatzis D, Stadler MB, Ciosk R.
Title The CSR-1 endogenous RNAi pathway ensures accurate transcriptional reprogramming during the oocyte-to-embryo transition in Caenorhabditis elegans.
Journal PLoS Genet
Abstract Endogenous RNAi (endoRNAi) is a conserved mechanism for fine-tuning gene expression. In the nematode Caenorhabditis elegans, several endoRNAi pathways are required for the successful development of reproductive cells. The CSR-1 endoRNAi pathway promotes germ cell development, primarily by facilitating the expression of germline genes. In this study, we report a novel function for the CSR-1 pathway in preventing premature activation of embryonic transcription in the developing oocytes, which is accompanied by a general Pol II activation. This CSR-1 function requires its RNase activity, suggesting that, by controlling the levels of maternal mRNAs, CSR-1-dependent endoRNAi contributes to an orderly reprogramming of transcription during the oocyte-to-embryo transition.
Volume 14(3)
Pages e1007252
Published 2018-3-1
DOI 10.1371/journal.pgen.1007252
PMID 29579041
PMC PMC5886687
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Embryo, Nonmammalian Female Gene Expression Regulation, Developmental Mutation Oocytes / physiology* RNA Interference* RNA Polymerase II / genetics RNA Polymerase II / metabolism Transcription Factors / genetics
IF 5.175
Times Cited 2
C.elegans tm892 tm1217