論文 - 詳細
| RRC ID | 53960 |
|---|---|
| 著者 | Hong Y, Sonneville R, Wang B, Scheidt V, Meier B, Woglar A, Demetriou S, Labib K, Jantsch V, Gartner A. |
| タイトル | LEM-3 is a midbody-tethered DNA nuclease that resolves chromatin bridges during late mitosis. |
| ジャーナル | Nat Commun |
| Abstract |
Faithful chromosome segregation and genome maintenance requires the removal of all DNA bridges that physically link chromosomes before cells divide. Using C. elegans embryos we show that the LEM-3/Ankle1 nuclease defines a previously undescribed genome integrity mechanism by processing DNA bridges right before cells divide. LEM-3 acts at the midbody, the structure where abscission occurs at the end of cytokinesis. LEM-3 localization depends on factors needed for midbody assembly, and LEM-3 accumulation is increased and prolonged when chromatin bridges are trapped at the cleavage plane. LEM-3 locally processes chromatin bridges that arise from incomplete DNA replication, unresolved recombination intermediates, or the perturbance of chromosome structure. Proper LEM-3 midbody localization and function is regulated by AIR-2/Aurora B kinase. Strikingly, LEM-3 acts cooperatively with the BRC-1/BRCA1 homologous recombination factor to promote genome integrity. These findings provide a molecular basis for the suspected role of the LEM-3 orthologue Ankle1 in human breast cancer. |
| 巻・号 | 9(1) |
| ページ | 728 |
| 公開日 | 2018-2-20 |
| DOI | 10.1038/s41467-018-03135-w |
| PII | 10.1038/s41467-018-03135-w |
| PMID | 29463814 |
| PMC | PMC5820297 |
| MeSH | Animals Caenorhabditis elegans / embryology Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Chromatin / genetics Chromatin / metabolism* Cytokinesis DNA / genetics DNA / metabolism DNA Replication Endodeoxyribonucleases / genetics Endodeoxyribonucleases / metabolism* Mitosis* |
| IF | 12.121 |
| 引用数 | 9 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3468 |