| RRC ID |
54029
|
| Author |
Sasa K, Yoshimura K, Yamada A, Suzuki D, Miyamoto Y, Imai H, Nagayama K, Maki K, Yamamoto M, Kamijo R.
|
| Title |
Monocarboxylate transporter-1 promotes osteoblast differentiation via suppression of p53, a negative regulator of osteoblast differentiation.
|
| Journal |
Sci Rep
|
| Abstract |
Monocarboxylate transporter-1 (MCT-1) is a transmembrane transporter for monocarboxylates including lactate and pyruvate. Silencing Mct1 by its small interfering RNA (siRNA) suppressed the expression of marker genes for osteoblast differentiation, namely, Tnap, Runx2, and Sp7, induced by BMP-2 in mouse myoblastic C2C12 cells. Mct1 siRNA also suppressed alkaline phosphatase activity, as well as expressions of Tnap and Bglap mRNAs in mouse primary osteoblasts. On the other hand, Mct1 siRNA did not have effects on the Smad1/5 or ERK/JNK pathways in BMP-2-stimulated C2C12 cells, while it up-regulated the mRNA expression of p53 (Trp53) as well as nuclear accumulation of p53 in C2C12 cells in a BMP-2-independent manner. Suppression of osteoblastic differentiation by Mct1 siRNA in C2C12 cells was abolished by co-transfection of Trp53 siRNA. Together, these results suggest that MCT-1 functions as a positive regulator of osteoblast differentiation via suppression of p53.
|
| Volume |
8(1)
|
| Pages |
10579
|
| Published |
2018-7-12
|
| DOI |
10.1038/s41598-018-28605-5
|
| PII |
10.1038/s41598-018-28605-5
|
| PMID |
30002387
|
| PMC |
PMC6043614
|
| MeSH |
Animals
Bone Morphogenetic Protein 2 / genetics
Bone Morphogenetic Protein 2 / metabolism
Cell Differentiation*
Cell Line
Gene Knockdown Techniques
Mice
Monocarboxylic Acid Transporters / genetics
Monocarboxylic Acid Transporters / metabolism*
Myoblasts / physiology*
Osteoblasts / physiology*
Primary Cell Culture
RNA, Small Interfering / metabolism
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Symporters / genetics
Symporters / metabolism*
Tumor Suppressor Protein p53 / metabolism*
|
| IF |
3.998
|
| Times Cited |
4
|
| Resource |
| Human and Animal Cells |
C2C12(RCB0987) |
