論文 - 詳細
| RRC ID | 54066 |
|---|---|
| 著者 | Ogiwara M, Ota W, Mizushige T, Kanamoto R, Ohinata K. |
| タイトル | Enzymatic digest of whey protein and wheylin-1, a dipeptide released in the digest, increase insulin sensitivity in an Akt phosphorylation-dependent manner. |
| ジャーナル | Food Funct |
| Abstract |
We investigated the effects of the enzymatic digest of β-lactoglobulin, a major bovine milk whey protein, on glucose metabolism in KK-Ay mice, an animal model of type II diabetes. In the glucose tolerance test and insulin tolerance test (ITT), the thermolysin digest of β-lactoglobulin decreased blood glucose levels, suggesting that it increases insulin sensitivity in diabetic KK-Ay mice. The digest also increased phosphorylation of Akt, an intracellular factor activated in response to the insulin receptor activation, in the liver and skeletal muscle. Next, we searched for a bioactive peptide present in the digest that increased the insulin sensitivity. Wheylin-1 is an anxiolytic-like dipeptide (Met-His) isolated from the thermolysin digest of β-lactoglobulin. Wheylin-1 decreased blood glucose levels in the ITT test and increased hepatic Akt phosphorylation. Wheylin-1 also increased insulin-induced Akt phosphorylation in hepatic HepG2 cells and muscular C2C12 myotube cells. These results suggest that wheylin-1 increases insulin sensitivity in an Akt-dependent manner in vivo and in vitro. Taken together, we found that the thermolysin digest of bovine milk whey β-lactoglobulin and wheylin-1 increase insulin sensitivity in an Akt system-dependent manner. Wheylin-1 is the first factor found that increases insulin sensitivity in association with Akt-phosphorylation. |
| 巻・号 | 9(9) |
| ページ | 4635-4641 |
| 公開日 | 2018-9-19 |
| DOI | 10.1039/c8fo00919h |
| PMID | 30151525 |
| MeSH | Animals Diabetes Mellitus, Type 2 / drug therapy* Diabetes Mellitus, Type 2 / genetics Diabetes Mellitus, Type 2 / metabolism Glucose Tolerance Test Humans Insulin / metabolism* Lactoglobulins / administration & dosage Lactoglobulins / chemistry* Liver / drug effects Liver / metabolism Male Muscle, Skeletal / drug effects Muscle, Skeletal / metabolism Peptide Fragments / administration & dosage* Peptide Fragments / chemistry Phosphorylation Proto-Oncogene Proteins c-akt / genetics Proto-Oncogene Proteins c-akt / metabolism* Thermolysin / chemistry Whey Proteins / chemistry |
| IF | 4.171 |
| 引用数 | 3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |