| RRC ID |
54068
|
| 著者 |
Toeda Y, Kasamatsu A, Koike K, Endo-Sakamoto Y, Fushimi K, Kasama H, Yamano Y, Shiiba M, Tanzawa H, Uzawa K.
|
| タイトル |
FBLIM1 enhances oral cancer malignancy via modulation of the epidermal growth factor receptor pathway.
|
| ジャーナル |
Mol Carcinog
|
| Abstract |
Filamin-binding LIM protein 1 (FBLIM1) is related to regulation of inflammatory responses, such as chronic recurrent multifocal osteomyelitis; however, the relevance of FBLIM1 in oral squamous cell carcinoma (OSCC) is unknown. The aim of the current study was to elucidate the possible role of FBLIM1 in the carcinogenesis of OSCC. We analyzed FBLIM1 expression using quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR), immunoblot analysis, and immunohistochemistry. The expression levels of FBLIM1 were up-regulated significantly (P < 0.05) in OSCC-derived cell lines and primary OSCCs specimens compared with normal counterparts. FBLIM1 expression also was correlated with the primary tumoral size (P < 0.05) and vascular invasion (P < 0.05). We then assessed tumoral progression after treatment with FBLIM1 siRNA and clopidogrel, an antiplatelet agent. Similar to the FBLIM1 knockdown effect, clopidogrel-treated cells had attenuated functions of proliferation, migration, and invasiveness. Interestingly, clopidogrel treatment led to down-regulation of epidermal growth factor receptor (EGFR) and FBLIM1. These findings identify FBLIM1 as a putative therapeutic target by using clopidogrel for inhibiting over activation of EGFR signaling to prevent OSCC malignancy.
|
| 巻・号 |
57(12)
|
| ページ |
1690-1697
|
| 公開日 |
2018-12-1
|
| DOI |
10.1002/mc.22889
|
| PMID |
30129678
|
| MeSH |
Aged
Aged, 80 and over
Carcinoma, Squamous Cell / genetics
Carcinoma, Squamous Cell / metabolism
Carcinoma, Squamous Cell / pathology*
Cell Adhesion Molecules / genetics*
Cell Adhesion Molecules / metabolism*
Cell Line, Tumor
Cell Movement / drug effects
Cell Proliferation / drug effects
Clopidogrel / pharmacology*
Cytoskeletal Proteins / genetics*
Cytoskeletal Proteins / metabolism*
ErbB Receptors / metabolism
Female
Gene Expression Regulation, Neoplastic
Humans
Male
Middle Aged
Mouth Neoplasms / genetics
Mouth Neoplasms / metabolism
Mouth Neoplasms / pathology*
Neoplasm Staging
Signal Transduction / drug effects*
Tumor Burden
Up-Regulation
|
| IF |
3.825
|
| 引用数 |
5
|
| リソース情報 |
| ヒト・動物細胞 |
HO-1-u-1(RCB2102) |
