| RRC ID |
5410
|
| 著者 |
Matsuda S, Miura E, Matsuda K, Kakegawa W, Kohda K, Watanabe M, Yuzaki M.
|
| タイトル |
Accumulation of AMPA receptors in autophagosomes in neuronal axons lacking adaptor protein AP-4.
|
| ジャーナル |
Neuron
|
| Abstract |
AP-4 is a member of the adaptor protein complexes, which control vesicular trafficking of membrane proteins. Although AP-4 has been suggested to contribute to basolateral sorting in epithelial cells, its function in neurons is unknown. Here, we show that disruption of the gene encoding the beta subunit of AP-4 resulted in increased accumulation of axonal autophagosomes, which contained AMPA receptors and transmembrane AMPA receptor regulatory proteins (TARPs), in axons of hippocampal neurons and cerebellar Purkinje cells both in vitro and in vivo. AP-4 indirectly associated with the AMPA receptor via TARPs, and the specific disruption of the interaction between AP-4 and TARPs caused the mislocalization of endogenous AMPA receptors in axons of wild-type neurons. These results indicate that AP-4 may regulate proper somatodendritic-specific distribution of its cargo proteins, including AMPA receptor-TARP complexes and the autophagic pathway in neurons.
|
| 巻・号 |
57(5)
|
| ページ |
730-45
|
| 公開日 |
2008-3-13
|
| DOI |
10.1016/j.neuron.2008.02.012
|
| PII |
S0896-6273(08)00135-9
|
| PMID |
18341993
|
| MeSH |
Adaptor Protein Complex 4 / deficiency*
Adaptor Protein Complex 4 / genetics
Amino Acid Sequence / genetics
Animals
Autophagy / physiology*
Axons / chemistry
Axons / physiology*
Cell Line
Cells, Cultured
Humans
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Molecular Sequence Data
Neurons / chemistry
Neurons / physiology
Phagosomes / chemistry
Phagosomes / physiology*
Rabbits
Receptors, AMPA / analysis
Receptors, AMPA / biosynthesis*
Receptors, AMPA / genetics
|
| IF |
14.415
|
| 引用数 |
91
|
|
WOS 分野
|
NEUROSCIENCES
|
| リソース情報 |
| 実験動物マウス |
RBRC00806 |
