| RRC ID |
5417
|
| 著者 |
Noda S, Horiguchi K, Ichikawa H, Miyoshi H.
|
| タイトル |
Repopulating activity of ex vivo-expanded murine hematopoietic stem cells resides in the CD48-c-Kit+Sca-1+lineage marker- cell population.
|
| ジャーナル |
Stem Cells
|
| Abstract |
A better understanding of the biology of cultured hematopoietic stem cells (HSCs) is required to achieve ex vivo expansion of HSCs. In this study, clonal analysis of the surface phenotype and repopulating activity of ex vivo-expanded murine HSCs was performed. After 7 days of culture with stem cell factor, thrombopoietin, fibroblast growth factor-1, and insulin-like growth factor-2, single CD34-/lowc-Kit+Sca-1+lineage marker- (CD34-KSL) cells gave rise to various numbers of cells. The proportion of KSL cells decreased with increasing number of expanded cells. Transplantation studies revealed that the progeny containing a higher percentage of KSL cells tended to have enhanced repopulating potential. We also found that CD48 was heterogeneously expressed in the KSL cell population after culture. Repopulating activity resided only in the CD48-KSL cell population, which had a relatively long intermitotic interval. Microarray analysis showed surprisingly few differences in gene expression between cultured CD48-KSL cells (cycling HSCs) and CD48+KSL cells (cycling non-HSCs) compared with freshly isolated CD34-KSL cells (quiescent HSCs), suggesting that the maintenance of stem cell activity is controlled by a relatively small number of genes. These findings should lead to a better understanding of ex vivo-expanded HSCs.
|
| 巻・号 |
26(3)
|
| ページ |
646-55
|
| 公開日 |
2008-3-1
|
| DOI |
10.1634/stemcells.2007-0623
|
| PII |
2007-0623
|
| PMID |
18079432
|
| MeSH |
Animals
Antigens, CD / metabolism*
Ataxin-1
Ataxins
Biomarkers / metabolism
CD48 Antigen
Cell Cycle / drug effects
Cell Lineage* / drug effects
Cell Proliferation / drug effects
Clone Cells
Cytokines / pharmacology
Gene Expression Profiling
Hematopoietic Stem Cells / cytology*
Hematopoietic Stem Cells / drug effects
Kinetics
Mice
Mice, Inbred C57BL
Nerve Tissue Proteins / metabolism*
Nuclear Proteins / metabolism*
Phenotype
Proto-Oncogene Proteins c-kit / metabolism*
|
| IF |
6.022
|
| 引用数 |
30
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
HEMATOLOGY
ONCOLOGY
CELL BIOLOGY
CELL & TISSUE ENGINEERING
|
| リソース情報 |
| 実験動物マウス |
RBRC00144 |
