| RRC ID |
54297
|
| 著者 |
Nishiura A, Kitagawa S, Matsumura M, Kazama Y, Abe T, Mizuno N, Nasuda S, Murai K.
|
| タイトル |
An early-flowering einkorn wheat mutant with deletions of PHYTOCLOCK 1/LUX ARRHYTHMO and VERNALIZATION 2 exhibits a high level of VERNALIZATION 1 expression induced by vernalization.
|
| ジャーナル |
J Plant Physiol
|
| Abstract |
Using heavy-ion beam mutagenesis of Triticum monococcum strain KU104-1, we identified a mutant that shows extra early-flowering; it was named extra early-flowering 3 (exe3). Here, we carried out expression analyses of clock-related genes, clock downstream genes and photoperiod pathway genes, and found that the clock component gene PHYTOCLOCK 1/LUX ARRHYTHMO (PCL1/LUX) was not expressed in exe3 mutant plants. A PCR analysis of DNA markers indicated that the exe3 mutant had a deletion of wheat PCL1/LUX (WPCL1), and that the WPCL1 deletion was correlated with the mutant phenotype in the segregation line. We confirmed that the original strain KU104-1 carried a mutation that produced a null allele of a flowering repressor gene VERNALIZATION 2 (VRN2). As a result, the exe3 mutant has both WPCL1 and VRN2 loss-of-function mutations. Analysis of plant development in a growth chamber showed that vernalization treatment accelerated flowering time in the exe3 mutant under short day (SD) as well as long day (LD) conditions, and the early-flowering phenotype was correlated with the earlier up-regulation of VRN1. The deletion of WPCL1 affects the SD-specific expression patterns of some clock-related genes, clock downstream genes and photoperiod pathway genes, suggesting that the exe3 mutant causes a disordered SD response. The present study indicates that VRN1 expression is associated with the biological clock and the VRN1 up-regulation is not influenced by the presence or absence of VRN2.
|
| 巻・号 |
222
|
| ページ |
28-38
|
| 公開日 |
2018-3-1
|
| DOI |
10.1016/j.jplph.2018.01.002
|
| PII |
S0176-1617(18)30009-9
|
| PMID |
29367015
|
| MeSH |
Flowers / genetics
Flowers / growth & development*
Gene Expression Regulation, Plant / genetics*
Genes, Plant / genetics*
Mutation
Photoperiod
Plant Proteins / metabolism
Sequence Deletion*
Triticum / genetics*
Triticum / growth & development
|
| IF |
2.825
|
| 引用数 |
3
|
| リソース情報 |
| コムギ |
|
