論文 - 詳細
| RRC ID | 54343 |
|---|---|
| 著者 | Tian L, Chou HL, Zhang L, Hwang SK, Starkenburg SR, Doroshenk KA, Kumamaru T, Okita TW. |
| タイトル | RNA-Binding Protein RBP-P Is Required for Glutelin and Prolamine mRNA Localization in Rice Endosperm Cells. |
| ジャーナル | Plant Cell |
| Abstract |
In developing rice (Oryza sativa) endosperm, mRNAs of the major storage proteins, glutelin and prolamine, are transported and anchored to distinct subdomains of the cortical endoplasmic reticulum. RNA binding protein RBP-P binds to both glutelin and prolamine mRNAs, suggesting a role in some aspect of their RNA metabolism. Here, we show that rice lines expressing mutant RBP-P mislocalize both glutelin and prolamine mRNAs. Different mutant RBP-P proteins exhibited varying degrees of reduced RNA binding and/or protein-protein interaction properties, which may account for the mislocalization of storage protein RNAs. In addition, partial loss of RBP-P function conferred a broad phenotypic variation ranging from dwarfism, chlorophyll deficiency, and sterility to late flowering and low spikelet fertility. Transcriptome analysis highlighted the essential role of RBP-P in regulating storage protein genes and several essential biological processes during grain development. Overall, our data demonstrate the significant roles of RBP-P in glutelin and prolamine mRNA localization and in the regulation of genes important for plant growth and development through its RNA binding activity and cooperative regulation with interacting proteins. |
| 巻・号 | 30(10) |
| ページ | 2529-2552 |
| 公開日 | 2018-10-1 |
| DOI | 10.1105/tpc.18.00321 |
| PII | tpc.18.00321 |
| PMID | 30190374 |
| PMC | PMC6241268 |
| MeSH | Endoplasmic Reticulum / genetics Endoplasmic Reticulum / metabolism Endosperm / genetics Endosperm / metabolism* Gene Expression Regulation, Plant Glutens / genetics* Glutens / metabolism Mutation Oryza / genetics Oryza / growth & development Oryza / metabolism* Prolamins / genetics* Prolamins / metabolism Protein Domains Protein Multimerization RNA, Messenger / metabolism RNA, Plant / metabolism RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* |
| IF | 9.618 |
| 引用数 | 9 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 16 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| イネ | Induced mutation line |