RRC ID |
54345
|
Author |
Nakahashi Y, Taketani S, Okuda M, Inoue K, Tokunaga R.
|
Title |
Molecular cloning and sequence analysis of cDNA encoding human ferrochelatase.
|
Journal |
Biochem Biophys Res Commun
|
Abstract |
The cDNA encoding human ferrochelatase [EC 4.99.1.1] was isolated from a human placenta cDNA library in bacteriophage lambda gt11 by screening with a radiolabeled fragment of mouse ferrochelatase cDNA. The cDNA had an open reading frame of 1269 base pairs (bp) encoding a protein of 423 amino acid residues (Mr. 47,833) with alternative putative polyadenylation signals in the 3' non-coding regions and poly (A) tails. Amino acid sequencing showed that the mature protein consists of 369 amino acid residues (Mr. 42,158) with a putative leader sequence of 54 amino acid residues. The human enzyme showed an 88% identity to mouse enzyme and 46% to yeast enzyme. Northern blot analysis showed two mRNAs of about 2500 and 1600 bp for ferrochelatase in K562 and HepG2 cells. As full-length cDNA for human ferrochelatase is now available, molecular lesions related to erythropoietic protoporphyria can be characterized.
|
Volume |
173(2)
|
Pages |
748-55
|
Published |
1990-12-14
|
DOI |
10.1016/s0006-291x(05)80099-3
|
PII |
S0006-291X(05)80099-3
|
PMID |
2260980
|
MeSH |
Amino Acid Sequence
Animals
Blotting, Northern
Cattle
Cloning, Molecular
DNA / biosynthesis*
Ferrochelatase / biosynthesis
Ferrochelatase / genetics*
Humans
Mice
Molecular Sequence Data
Open Reading Frames
RNA, Messenger / chemistry
Restriction Mapping
Sequence Homology, Nucleic Acid
Tumor Cells, Cultured
|
IF |
2.985
|
Times Cited |
148
|
Resource |
DNA material |
ferrochelatase (RDB01377) |