論文 - 詳細
| RRC ID | 54345 |
|---|---|
| 著者 | Nakahashi Y, Taketani S, Okuda M, Inoue K, Tokunaga R. |
| タイトル | Molecular cloning and sequence analysis of cDNA encoding human ferrochelatase. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
The cDNA encoding human ferrochelatase [EC 4.99.1.1] was isolated from a human placenta cDNA library in bacteriophage lambda gt11 by screening with a radiolabeled fragment of mouse ferrochelatase cDNA. The cDNA had an open reading frame of 1269 base pairs (bp) encoding a protein of 423 amino acid residues (Mr. 47,833) with alternative putative polyadenylation signals in the 3' non-coding regions and poly (A) tails. Amino acid sequencing showed that the mature protein consists of 369 amino acid residues (Mr. 42,158) with a putative leader sequence of 54 amino acid residues. The human enzyme showed an 88% identity to mouse enzyme and 46% to yeast enzyme. Northern blot analysis showed two mRNAs of about 2500 and 1600 bp for ferrochelatase in K562 and HepG2 cells. As full-length cDNA for human ferrochelatase is now available, molecular lesions related to erythropoietic protoporphyria can be characterized. |
| 巻・号 | 173(2) |
| ページ | 748-55 |
| 公開日 | 1990-12-14 |
| DOI | 10.1016/s0006-291x(05)80099-3 |
| PII | S0006-291X(05)80099-3 |
| PMID | 2260980 |
| MeSH | Amino Acid Sequence Animals Blotting, Northern Cattle Cloning, Molecular DNA / biosynthesis* Ferrochelatase / biosynthesis Ferrochelatase / genetics* Humans Mice Molecular Sequence Data Open Reading Frames RNA, Messenger / chemistry Restriction Mapping Sequence Homology, Nucleic Acid Tumor Cells, Cultured |
| IF | 2.985 |
| 引用数 | 148 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | ferrochelatase (RDB01377) |