RRC ID 54345
Author Nakahashi Y, Taketani S, Okuda M, Inoue K, Tokunaga R.
Title Molecular cloning and sequence analysis of cDNA encoding human ferrochelatase.
Journal Biochem Biophys Res Commun
Abstract The cDNA encoding human ferrochelatase [EC 4.99.1.1] was isolated from a human placenta cDNA library in bacteriophage lambda gt11 by screening with a radiolabeled fragment of mouse ferrochelatase cDNA. The cDNA had an open reading frame of 1269 base pairs (bp) encoding a protein of 423 amino acid residues (Mr. 47,833) with alternative putative polyadenylation signals in the 3' non-coding regions and poly (A) tails. Amino acid sequencing showed that the mature protein consists of 369 amino acid residues (Mr. 42,158) with a putative leader sequence of 54 amino acid residues. The human enzyme showed an 88% identity to mouse enzyme and 46% to yeast enzyme. Northern blot analysis showed two mRNAs of about 2500 and 1600 bp for ferrochelatase in K562 and HepG2 cells. As full-length cDNA for human ferrochelatase is now available, molecular lesions related to erythropoietic protoporphyria can be characterized.
Volume 173(2)
Pages 748-55
Published 1990-12-14
DOI 10.1016/s0006-291x(05)80099-3
PII S0006-291X(05)80099-3
PMID 2260980
MeSH Amino Acid Sequence Animals Blotting, Northern Cattle Cloning, Molecular DNA / biosynthesis* Ferrochelatase / biosynthesis Ferrochelatase / genetics* Humans Mice Molecular Sequence Data Open Reading Frames RNA, Messenger / chemistry Restriction Mapping Sequence Homology, Nucleic Acid Tumor Cells, Cultured
IF 2.705
Times Cited 148
Resource
DNA material ferrochelatase (RDB01377)