論文 - 詳細
| RRC ID | 54355 |
|---|---|
| 著者 | MaruYama T, Kobayashi S, Ogasawara K, Yoshimura A, Chen W, Muta T. |
| タイトル | Control of IFN-γ production and regulatory function by the inducible nuclear protein IκB-ζ in T cells. |
| ジャーナル | J Leukoc Biol |
| Abstract |
The transcriptional regulator IκB-ζ is important for the control of apoptosis in keratinocytes. Thus, IκB-ζ-deficient mice develop autoimmune diseases, such as Sjögren's syndrome. However, T cells also play a pivotal role in Sjögren's syndrome. To study the role of IκB-ζ in T cells, we generated T cell-specific, IκB-ζ-deficient mice. We observed increased numbers of peripheral effector/memory CD4(+) cells and IFN-γ-producing CD4(+) cells in 3-week-old mice. We found that IκB-ζ can be up-regulated by TGF-β1 in naïve CD4(+) T cells and that it negatively regulates IFN-γ expression. In addition, we generated Treg-specific, IκB-ζ deficient mice and found that IκB-ζ is dispensable for the plasticity and stability of Tregs. However, Tregs from T cell-specific, IκB-ζ-deficient mice have reduced immunoregulatory function. Thus, our data reveal a previously unappreciated role for IκB-ζ in IFN-γ production in T cells and the immunoregulatory function of Tregs. |
| 巻・号 | 98(3) |
| ページ | 385-93 |
| 公開日 | 2015-9-1 |
| DOI | 10.1189/jlb.2A0814-384R |
| PII | jlb.2A0814-384R |
| PMID | 26019294 |
| PMC | PMC4541499 |
| MeSH | Adaptor Proteins, Signal Transducing / deficiency Adaptor Proteins, Signal Transducing / metabolism* Animals Colitis / chemically induced Colitis / immunology Colitis / pathology Forkhead Transcription Factors / genetics Forkhead Transcription Factors / metabolism Gene Expression Regulation / drug effects HEK293 Cells Homeostasis / drug effects Humans Immune Tolerance / drug effects Interferon-gamma / biosynthesis* Interferon-gamma / genetics Mice, Inbred C57BL Mice, Knockout Nuclear Proteins / deficiency Nuclear Proteins / metabolism* T-Lymphocytes, Regulatory / drug effects T-Lymphocytes, Regulatory / immunology T-Lymphocytes, Regulatory / metabolism* Transforming Growth Factor beta1 / pharmacology |
| IF | 4.224 |
| 引用数 | 6 |
| リソース情報 | |
| 遺伝子材料 | pGL4.12-mouse IFN-g promoter(600bp) (RDB16732) pcDNA3-FLAG-mouse IkappaB-zeta(L) (RDB16730) pcDNA3-FLAG-mouse IkappaB-zeta(S) (RDB16731) pMY-FLAG-mouse IkB-zeta(L)-IRES-GFP (RDB16733) pMY-FLAG-mouse IkB-zeta(S)-IRES-GFP (RDB16734) |