RRC ID 54360
著者 Thakran P, Pandit PA, Datta S, Kolathur KK, Pleiss JA, Mishra SK.
タイトル Sde2 is an intron-specific pre-mRNA splicing regulator activated by ubiquitin-like processing.
ジャーナル EMBO J
Abstract The expression of intron-containing genes in eukaryotes requires generation of protein-coding messenger RNAs (mRNAs) via RNA splicing, whereby the spliceosome removes non-coding introns from pre-mRNAs and joins exons. Spliceosomes must ensure accurate removal of highly diverse introns. We show that Sde2 is a ubiquitin-fold-containing splicing regulator that supports splicing of selected pre-mRNAs in an intron-specific manner in Schizosaccharomyces pombe Both fission yeast and human Sde2 are translated as inactive precursor proteins harbouring the ubiquitin-fold domain linked through an invariant GGKGG motif to a C-terminal domain (referred to as Sde2-C). Precursor processing after the first di-glycine motif by the ubiquitin-specific proteases Ubp5 and Ubp15 generates a short-lived activated Sde2-C fragment with an N-terminal lysine residue, which subsequently gets incorporated into spliceosomes. Absence of Sde2 or defects in Sde2 activation both result in inefficient excision of selected introns from a subset of pre-mRNAs. Sde2 facilitates spliceosomal association of Cactin/Cay1, with a functional link between Sde2 and Cactin further supported by genetic interactions and pre-mRNA splicing assays. These findings suggest that ubiquitin-like processing of Sde2 into a short-lived activated form may function as a checkpoint to ensure proper splicing of certain pre-mRNAs in fission yeast.
巻・号 37(1)
ページ 89-101
公開日 2018-1-4
DOI 10.15252/embj.201796751
PII embj.201796751
PMID 28947618
PMC PMC5753039
MeSH DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Genomic Instability Humans Introns* RNA Precursors / genetics RNA Splicing* RNA, Fungal / genetics RNA, Fungal / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Spliceosomes Ubiquitin / metabolism*
IF 9.889
引用数 10
リソース情報
酵母 FY23646 FY23631 FY23639