論文 - 詳細
| RRC ID | 54360 |
|---|---|
| 著者 | Thakran P, Pandit PA, Datta S, Kolathur KK, Pleiss JA, Mishra SK. |
| タイトル | Sde2 is an intron-specific pre-mRNA splicing regulator activated by ubiquitin-like processing. |
| ジャーナル | EMBO J |
| Abstract |
The expression of intron-containing genes in eukaryotes requires generation of protein-coding messenger RNAs (mRNAs) via RNA splicing, whereby the spliceosome removes non-coding introns from pre-mRNAs and joins exons. Spliceosomes must ensure accurate removal of highly diverse introns. We show that Sde2 is a ubiquitin-fold-containing splicing regulator that supports splicing of selected pre-mRNAs in an intron-specific manner in Schizosaccharomyces pombe Both fission yeast and human Sde2 are translated as inactive precursor proteins harbouring the ubiquitin-fold domain linked through an invariant GGKGG motif to a C-terminal domain (referred to as Sde2-C). Precursor processing after the first di-glycine motif by the ubiquitin-specific proteases Ubp5 and Ubp15 generates a short-lived activated Sde2-C fragment with an N-terminal lysine residue, which subsequently gets incorporated into spliceosomes. Absence of Sde2 or defects in Sde2 activation both result in inefficient excision of selected introns from a subset of pre-mRNAs. Sde2 facilitates spliceosomal association of Cactin/Cay1, with a functional link between Sde2 and Cactin further supported by genetic interactions and pre-mRNA splicing assays. These findings suggest that ubiquitin-like processing of Sde2 into a short-lived activated form may function as a checkpoint to ensure proper splicing of certain pre-mRNAs in fission yeast. |
| 巻・号 | 37(1) |
| ページ | 89-101 |
| 公開日 | 2018-1-4 |
| DOI | 10.15252/embj.201796751 |
| PII | embj.201796751 |
| PMID | 28947618 |
| PMC | PMC5753039 |
| MeSH | DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Genomic Instability Humans Introns* RNA Precursors / genetics RNA Splicing* RNA, Fungal / genetics RNA, Fungal / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Spliceosomes Ubiquitin / metabolism* |
| IF | 9.889 |
| 引用数 | 10 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY23646 FY23631 FY23639 |