論文 - 詳細
| RRC ID | 54369 |
|---|---|
| 著者 | Yin Y, Borges G, Sakuta M, Crozier A, Ashihara H. |
| タイトル | Effect of phosphate deficiency on the content and biosynthesis of anthocyanins and the expression of related genes in suspension-cultured grape (Vitis sp.) cells. |
| ジャーナル | Plant Physiol Biochem |
| Abstract |
The effect of phosphate (Pi) starvation on anthocyanin biosynthesis was examined in suspension-cultured grape cells derived from Bailey Alicante A grapes. Cyanindin-3-O-glucoside, peonidin-3-O-glucoside, cyanidin-3-O-(6-O״-p-coumaroyl)-glucoside and peonidin-3-O-(6-O״-p-coumaroyl)-glucoside were detected in cells in both the complete (+Pi)- and Pi-deficient Murashige-Skoog (-Pi) medium but accumulation was much more substantial cells in -Pi cultures. Highest incorporation of [ring-(14)C]phenylalanine into anthocyanins was found at days 3-14 in the cells in -Pi medium. Time-course expression profiles of anthocyanin biosynthetic enzyme genes and a regulatory gene were examined after cells were transferred to the +Pi and -Pi medium. The transcript levels of UFGT and VvmybA1 in -Pi cells were higher than in +Pi cells 24 h after transfer coinciding with the start of anthocyanin accumulation in -Pi cells. |
| 巻・号 | 55 |
| ページ | 77-84 |
| 公開日 | 2012-6-1 |
| DOI | 10.1016/j.plaphy.2012.03.009 |
| PII | S0981-9428(12)00066-6 |
| PMID | 22554830 |
| MeSH | Anthocyanins / biosynthesis* Cells, Cultured Chromatography, High Pressure Liquid Culture Media / metabolism Culture Media / pharmacology Gene Expression Regulation, Plant / drug effects* Glucosyltransferases / genetics Glucosyltransferases / metabolism Mass Spectrometry Phenylalanine / metabolism Phosphates / metabolism Phosphates / pharmacology* Plant Proteins / genetics* Plant Proteins / metabolism Reverse Transcriptase Polymerase Chain Reaction Time Factors Vitis / cytology Vitis / genetics Vitis / metabolism |
| IF | 3.72 |
| 引用数 | 12 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00003 |