Reference - Detail
| RRC ID | 54403 |
|---|---|
| Author | Tsurumi Y, Hamada Y, Katoh Y, Nakayama K. |
| Title | Interactions of the dynein-2 intermediate chain WDR34 with the light chains are required for ciliary retrograde protein trafficking. |
| Journal | Mol Biol Cell |
| Abstract |
The dynein-2 complex drives retrograde ciliary protein trafficking by associating with the intraflagellar transport (IFT) machinery, containing IFT-A and IFT-B complexes. We recently showed that the dynein-2 complex, which comprises 11 subunits, can be divided into three subcomplexes: DYNC2H1-DYNC2LI1, WDR34-DYNLL1/DYNLL2-DYNLRB1/DYNLRB2, and WDR60-TCTEX1D2-DYNLT1/DYNLT3. In this study, we demonstrated that the WDR34 intermediate chain interacts with the two light chains, DYNLL1/DYNLL2 and DYNLRB1/DYNLRB2, via its distinct sites. Phenotypic analyses of WDR34-knockout cells exogenously expressing various WDR34 constructs showed that the interactions of the WDR34 intermediate chain with the light chains are crucial for ciliary retrograde protein trafficking. Furthermore, we found that expression of the WDR34 N-terminal construct encompassing the light chain-binding sites but lacking the WD40 repeat domain inhibits ciliary biogenesis and retrograde trafficking in a dominant-negative manner, probably by sequestering WDR60 or the light chains. Taken together with phenotypic differences of several WDR34-knockout cell lines, these results indicate that incorporation of DYNLL1/DYNLL2 and DYNLRB1/DYNLRB2 into the dynein-2 complex via interactions with the WDR34 intermediate chain is crucial for dynein-2 function in retrograde ciliary protein trafficking. |
| Volume | 30(5) |
| Pages | 658-670 |
| Published | 2019-3-1 |
| DOI | 10.1091/mbc.E18-10-0678 |
| PMID | 30649997 |
| PMC | PMC6589695 |
| MeSH | Amino Acid Sequence Binding Sites Carrier Proteins / chemistry Carrier Proteins / metabolism* Cilia / metabolism* Dyneins / chemistry Dyneins / metabolism* HEK293 Cells Humans Protein Binding Protein Transport |
| IF | 3.791 |
| Times Cited | 7 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | Genome Network Project Human cDNA Clone IRAL028A16 (HGY091216) |