論文 - 詳細
| RRC ID | 54430 |
|---|---|
| 著者 | Misra JR, Irvine KD. |
| タイトル | Early girl is a novel component of the Fat signaling pathway. |
| ジャーナル | PLoS Genet |
| Abstract |
The Drosophila protocadherins Dachsous and Fat regulate growth and tissue polarity by modulating the levels, membrane localization and polarity of the atypical myosin Dachs. Localization to the apical junctional membrane is critical for Dachs function, and the adapter protein Vamana/Dlish and palmitoyl transferase Approximated are required for Dachs membrane localization. However, how Dachs levels are regulated is poorly understood. Here we identify the early girl gene as playing an essential role in Fat signaling by limiting the levels of Dachs protein. early girl mutants display overgrowth of the wings and reduced cross vein spacing, hallmark features of mutations affecting Fat signaling. Genetic experiments reveal that it functions in parallel with Fat to regulate Dachs. early girl encodes an E3 ubiquitin ligase, physically interacts with Dachs, and regulates its protein stability. Concomitant loss of early girl and approximated results in accumulation of Dachs and Vamana in cytoplasmic punctae, suggesting that it also regulates their trafficking to the apical membrane. Our findings establish a crucial role for early girl in Fat signaling, involving regulation of Dachs and Vamana, two key downstream effectors of this pathway. |
| 巻・号 | 15(1) |
| ページ | e1007955 |
| 公開日 | 2019-1-1 |
| DOI | 10.1371/journal.pgen.1007955 |
| PII | PGENETICS-D-18-01775 |
| PMID | 30699121 |
| PMC | PMC6370246 |
| MeSH | Acyltransferases / genetics* Animals Cell Adhesion Molecules / genetics* Cell Polarity / genetics Drosophila Proteins / genetics* Drosophila melanogaster / genetics Humans Membrane Proteins / genetics Mutation Myosins / genetics Protein Transport / genetics Signal Transduction Ubiquitin-Protein Ligases / genetics* Wings, Animal / growth & development |
| IF | 5.224 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 17033R-3 |