Reference - Detail
| RRC ID | 54463 |
|---|---|
| Author | Matsumoto M, Kikkawa S, Kohase M, Miyake K, Seya T. |
| Title | Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
A monoclonal antibody (mAb) against human Toll-like receptor (TLR) 3 was established and its effect on TLR3-mediated responses was tested using human fibroblast cell lines expressing TLR3 on the cell surface. Fibroblasts are known to produce IFN-beta upon viral infection or treatment with double-stranded RNA (dsRNA) through distinct signaling pathways. Here, we show the mAb to TLR3 suppressed poly(I):poly(C)-mediated IFN-beta production by human fibroblasts naturally expressing TLR3 on their surface. By reporter gene assay using HEK293 cells transfected with a human TLR3 expression vector, TLR3 recognized dsRNA to activate NF-kappaB and the IFN-beta promoter. TLR3 signaling was not elicited by either single-stranded RNA (ssRNA) or dsDNA. Thus, specific recognition of dsRNA by extracellular TLR3 is essential for induction of type I IFN: the interassociation between dsRNA and TLR3, regardless of direct or indirect binding, should be disrupted by mAb being attached to TLR3. The mAb against TLR3 reported herein may serve as a regulator for virus-mediated immune response via an alternative pathway involving the dsRNA-TLR3 recognition which might occur on host cells. |
| Volume | 293(5) |
| Pages | 1364-9 |
| Published | 2002-5-24 |
| DOI | 10.1016/S0006-291X(02)00380-7 |
| PII | S0006-291X(02)00380-7 |
| PMID | 12054664 |
| MeSH | Animals Antibodies, Monoclonal / metabolism* Cell Line Cell Membrane / metabolism DNA, Complementary / metabolism Dose-Response Relationship, Drug Drosophila Proteins* Fibroblasts / metabolism Flow Cytometry Genes, Reporter Genetic Vectors Humans Interferon-beta / metabolism Luciferases / metabolism Lung / metabolism Membrane Glycoproteins / chemistry Membrane Glycoproteins / immunology* Mice Mice, Inbred BALB C NF-kappa B / metabolism Promoter Regions, Genetic Protein Binding RNA / metabolism* RNA, Double-Stranded / metabolism Receptors, Cell Surface / chemistry Receptors, Cell Surface / immunology* Recombinant Proteins / metabolism Signal Transduction Toll-Like Receptor 3 Toll-Like Receptors Transfection |
| IF | 2.985 |
| Times Cited | 340 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 52 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | human TLR3 expression vector (RDB16909) human TLR3-CFlag expression vector (RDB16911) |
| Human and Animal Cells | MRC-5 |