RRC ID 54489
著者 Ford MJ, Yeyati PL, Mali GR, Keighren MA, Waddell SH, Mjoseng HK, Douglas AT, Hall EA, Sakaue-Sawano A, Miyawaki A, Meehan RR, Boulter L, Jackson IJ, Mill P, Mort RL.
タイトル A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice.
ジャーナル Dev Cell
Abstract The cilia and cell cycles are inextricably linked. Centrioles in the basal body of cilia nucleate the ciliary axoneme and sequester pericentriolar matrix (PCM) at the centrosome to organize the mitotic spindle. Cilia themselves respond to growth signals, prompting cilia resorption and cell cycle re-entry. We describe a fluorescent cilia and cell cycle biosensor allowing live imaging of cell cycle progression and cilia assembly and disassembly kinetics in cells and inducible mice. We define assembly and disassembly in relation to cell cycle stage with single-cell resolution and explore the intercellular heterogeneity in cilia kinetics. In all cells and tissues analyzed, we observed cilia that persist through the G1/S transition and into S/G2/M-phase. We conclude that persistence of cilia after the G1/S transition is a general property. This resource will shed light at an individual cell level on the interplay between the cilia and cell cycles in development, regeneration, and disease.
巻・号 47(4)
ページ 509-523.e5
公開日 2018-11-19
DOI 10.1016/j.devcel.2018.10.027
PII S1534-5807(18)30881-5
PMID 30458140
PMC PMC6251972
MeSH Animals Basal Bodies / metabolism Biosensing Techniques / methods Cell Cycle / physiology* Cell Cycle Proteins / metabolism Centrioles / metabolism* Centrosome / metabolism* Cilia / metabolism* Kinetics Mice Microtubules / metabolism
IF 9.19
引用数 15
リソース情報
遺伝子材料 pCDNA5-Frt-CAG-Arl13bCerulean-Fucci2a (RDB16057) pRosa26-CAG-floxNeo-Arl13bCerulean-Fucci2a (RDB16058) pCAG-Arl13bCerulean-Fucci2a (RDB16059) pCAG-Fucci2a (RDB13080) pROSA-floxNeo-Fucci2a (RDB13081)