論文 - 詳細
| RRC ID | 54492 |
|---|---|
| 著者 | Pahi Z, Borsos BN, Vedelek B, Shidlovskii YV, Georgieva SG, Boros IM, Pankotai T. |
| タイトル | TAF10 and TAF10b partially redundant roles during Drosophila melanogaster morphogenesis. |
| ジャーナル | Transcription |
| Abstract |
Transcription of eukaryotic genes requires the cooperative action of the RNA polymerase complex, the general transcription factors (TFIIB, TFIID, TFIIE, TFIIF and TFIIH) and chromatin modifiers. The TFIID complex contributes to transcriptional activation by several mechanisms and has a subunit with associated histone acetyltransferase (HAT) activity. The histone modifier SAGA complex has both HAT and deubiquitylase (DUB) activities. TFIID and SAGA share several TBP-associated factors (TAFs), but not their HAT subunit. Recently, several duplicated TAF proteins have been identified in higher eukaryotes, but their functional diversity has been so far poorly characterized. Here, we report the functional similarities and differences of TAF10 and TAF10b, the two TAF10 orthologs of Drosophila melanogaster. Results from in silico modeling suggest that dTAF10 and dTAF10b have similar secondary structures characterized by the presence of a histone-fold domain. Additionally, dTAF10 and dTAF10b share interaction partners and show similar expression patterns in neuronal tissues. Nonetheless, dTAF10 and dTAF10b seem to have partly distinct functions. To investigate their roles, we generated dTaf10-dTaf10b double-mutants and rescued the mutant flies with transgenes, which allowed the translation of either dTAF10 or dTAF10b protein. We found that the loss of dTAF10b resulted in pupal lethality, while animals lacking dTAF10 were able to form puparium. dTaf10 mutant adults showed distorted eye morphology. During DNA repair, dTAF10 and dTAF10b act redundantly, suggesting that these proteins have distinct but partially overlapping functions. |
| 巻・号 | 8(5) |
| ページ | 297-306 |
| 公開日 | 2017-8-25 |
| DOI | 10.1080/21541264.2017.1327836 |
| PMID | 28841365 |
| PMC | PMC5703247 |
| MeSH | Animals Computer Simulation DNA Repair Drosophila Proteins / chemistry* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / chemistry Drosophila melanogaster / genetics Drosophila melanogaster / growth & development* Drosophila melanogaster / metabolism Eye / anatomy & histology Eye / metabolism Gene Expression Regulation, Developmental Models, Molecular Morphogenesis Mutation Neurons / metabolism Protein Domains Protein Structure, Secondary TATA-Binding Protein Associated Factors / chemistry* TATA-Binding Protein Associated Factors / genetics TATA-Binding Protein Associated Factors / metabolism* Transcription Factor TFIID / chemistry* Transcription Factor TFIID / genetics Transcription Factor TFIID / metabolism* |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 2859R-5 |