RRC ID 54588
Author Syrzycka M, Hallson G, Fitzpatrick KA, Kim I, Cotsworth S, Hollebakken RE, Simonetto K, Yang L, Luongo S, Beja K, Coulthard AB, Hilliker AJ, Sinclair DA, Honda BM.
Title Genetic and Molecular Analysis of Essential Genes in Centromeric Heterochromatin of the Left Arm of Chromosome 3 in Drosophila melanogaster.
Journal G3 (Bethesda)
Abstract A large portion of the Drosophila melanogaster genome is contained within heterochromatic regions of chromosomes, predominantly at centromeres and telomeres. The remaining euchromatic portions of the genome have been extensively characterized with respect to gene organization, function and regulation. However, it has been difficult to derive similar data for sequences within centromeric (centric) heterochromatin because these regions have not been as amenable to analysis by standard genetic and molecular tools. Here we present an updated genetic and molecular analysis of chromosome 3L centric heterochromatin (3L Het). We have generated and characterized a number of new, overlapping deficiencies (Dfs) which remove regions of 3L Het. These Dfs were critically important reagents in our subsequent genetic analysis for the isolation and characterization of lethal point mutations in the region. The assignment of these mutations to genetically-defined essential loci was followed by matching them to gene models derived from genome sequence data: this was done by using molecular mapping plus sequence analysis of mutant alleles, thereby aligning genetic and physical maps of the region. We also identified putative essential gene sequences in 3L Het by using RNA interference to target candidate gene sequences. We report that at least 25, or just under 2/3 of loci in 3L Het, are essential for viability and/or fertility. This work contributes to the functional annotation of centric heterochromatin in Drosophila, and the genetic and molecular tools generated should help to provide important insights into the organization and functions of gene sequences in 3L Het.
Volume 9(5)
Pages 1581-1595
Published 2019-5-7
DOI 10.1534/g3.119.0003
PII g3.119.0003
PMID 30948422
PMC PMC6505167
MeSH Animals Centromere / genetics* Chromosome Mapping Chromosomes, Insect* Drosophila melanogaster / genetics* Gene Knockdown Techniques Genes, Essential* Genes, Lethal Genetic Complementation Test Genetic Testing Genomics* / methods Genotype Heterochromatin / genetics* Models, Genetic Mutagenesis / radiation effects Mutation RNA Interference X-Rays
IF 2.63
Times Cited 2