| RRC ID |
54609
|
| Author |
Rajniak J, Giehl RFH, Chang E, Murgia I, von Wirén N, Sattely ES.
|
| Title |
Biosynthesis of redox-active metabolites in response to iron deficiency in plants.
|
| Journal |
Nat Chem Biol
|
| Abstract |
Iron is an essential but poorly bioavailable nutrient because of its low solubility, especially in alkaline soils. Here, we describe the discovery of a previously undescribed redox-active catecholic metabolite, termed sideretin, which derives from the coumarin fraxetin and is the primary molecule exuded by Arabidopsis thaliana roots in response to iron deficiency. We identified two enzymes that complete the biosynthetic pathway of fraxetin and sideretin. Chemical characterization of fraxetin and sideretin, and biological assays with pathway mutants, suggest that these coumarins are critical for iron nutrition in A. thaliana. Further, we show that sideretin production also occurs in eudicot species only distantly related to A. thaliana. Untargeted metabolomics of the root exudates of various eudicots revealed production of structurally diverse redox-active molecules in response to iron deficiency. Our results indicate that secretion of small-molecule reductants by roots may be a widespread and previously underappreciated component of reduction-based iron uptake.
|
| Volume |
14(5)
|
| Pages |
442-450
|
| Published |
2018-5-1
|
| DOI |
10.1038/s41589-018-0019-2
|
| PII |
10.1038/s41589-018-0019-2
|
| PMID |
29581584
|
| PMC |
PMC6693505
|
| MeSH |
Arabidopsis / metabolism*
Arabidopsis Proteins / metabolism
Coumarins / metabolism*
Gene Expression Regulation, Plant
Genetic Complementation Test
Iron Deficiencies*
Kinetics
Metabolomics
Mutation
Oxidation-Reduction*
Phenotype
Phylogeny
Plant Roots / metabolism*
Plants, Genetically Modified
Rhizosphere
Scopoletin / metabolism
Solubility
Thermodynamics
|
| IF |
12.154
|
| Times Cited |
51
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
pda08472 |
