RRC ID 54614
Author Jaroensanti-Tanaka N, Miyazaki S, Hosoi A, Tanaka K, Ito S, Iuchi S, Nakano T, Kobayashi M, Nakajima M, Asami T.
Title The chemical NJ15 affects hypocotyl elongation and shoot gravitropism via cutin polymerization.
Journal Biosci Biotechnol Biochem
Abstract We previously found a chemical, designated as NJ15, which inhibited both auxin and brassinosteroid responses in dark-grown Arabidopsis. To study its mode of action, we performed a phenotypic screening of NJ15-low-sensitive lines among mutant pools of Arabidopsis. One line (f127) showed clear NJ15-low-sensitivity in terms of hypocotyl elongation and shoot gravitropism. After further testing, it was determined that DCR, an enzyme involved in cutin polymerization, had lost its function in the mutant, which caused its low sensitivity to NJ15. Fatty acids are the base materials for polymers such as cutin and cuticular wax. We confirmed that NJ15 affects fatty acid biosynthesis, and that it does differently from cafenstrole, a known inhibitor of cuticular wax formation. Based on these results, we propose that the target of NJ15 is likely located within the cutin polymer formation pathway.
ABBREVIATIONS:Caf: cafenstrole; DEG: differentially expressed gene; FDR: false discovery rate; FOX: full length cDNA-overexpressor; VLCFA: very-long-chain fatty acid.
Volume 82(10)
Pages 1770-1779
Published 2018-10-1
DOI 10.1080/09168451.2018.1484278
PMID 29912637
MeSH Arabidopsis / drug effects* Arabidopsis / genetics Arabidopsis / growth & development Fatty Acids / metabolism Gravitropism / drug effects* Hypocotyl / drug effects* Hypocotyl / growth & development Membrane Lipids / metabolism* Mutation Plant Shoots / drug effects* Plant Shoots / growth & development Polymerization Real-Time Polymerase Chain Reaction Sequence Analysis, RNA Sulfones / pharmacology Transcriptome Triazoles / pharmacology
IF 1.297
Times Cited 1
Arabidopsis / Cultured plant cells, genes pss10001