RRC ID 54618
Author Kundu A, Das S, Basu S, Kobayashi Y, Kobayashi Y, Koyama H, Ganesan M.
Title GhSTOP1, a C2H2 type zinc finger transcription factor is essential for aluminum and proton stress tolerance and lateral root initiation in cotton.
Journal Plant Biol (Stuttg)
Abstract Aluminum (Al) and proton (H+ ) ions are major acid soil stress factors deleteriously affecting plant root growth and crop yield. In our preliminary studies, cotton (Gossypium hirsutum L.) seedlings displayed very sensitive phenotypes to Al and H+ rhizotoxicities. Based on previous Arabidopsis results, we aimed to characterise the Al stress responsive Sensitive to Proton rhizotoxicity 1 (GhSTOP1) transcription system in cotton using RNAi-mediated down-regulation. With the help of seed embryo apex explants, we developed transgenic cotton plants overexpressing a GhSTOP1-RNAi cassette with NPTII selection. Kanamycin-tolerant T1 seedlings were further considered for Al and H+ stress tolerance studies. Down-regulation of the GhSTOP1 increased sensitivity to Al and proton rhizotoxicities, and root growth was significantly reduced in RNAi lines. The expression profile of GhALMT1 (Aluminum-activated Malate Transporter 1), GhMATE (Multidrug and Toxic Compound Extrusion), GhALS3 (Aluminium Sensitive 3) and key genes involved in the GABA shunt were down-regulated in the transgenic RNAi lines. Additionally, the lateral root initiation process was delayed and expression of GhNAC1, which is involved in lateral roots, was also suppressed in transgenic lines. Besides, overexpression of GhSTOP1 in Arabidopsis accelerated root growth and AtMATE and AtALMT1 expression under Al stress conditions. These analyses indicate that GhSTOP1 is essential for the expression of several genes which are necessary for acid soil tolerance mechanisms and lateral root initiation.
Volume 21(1)
Pages 35-44
Published 2019-1-1
DOI 10.1111/plb.12895
PMID 30098101
MeSH Adaptation, Physiological / drug effects* Aluminum / toxicity* Amino Acid Sequence Arabidopsis / genetics Base Sequence Citrates / metabolism Cloning, Molecular Down-Regulation / drug effects Down-Regulation / genetics Gene Expression Regulation, Plant / drug effects Genes, Plant Gossypium / physiology* Malates / metabolism Phenotype Phylogeny Plant Proteins / chemistry Plant Proteins / genetics Plant Proteins / metabolism* Plant Roots / drug effects Plant Roots / physiology* Plants, Genetically Modified Protons* RNA Interference RNA, Messenger / genetics RNA, Messenger / metabolism Soil Stress, Physiological / drug effects Transcription Factors / chemistry Transcription Factors / metabolism* Zinc Fingers*
IF 2.393
Times Cited 6
Arabidopsis / Cultured plant cells, genes sja05800