| RRC ID |
54618
|
| Author |
Kundu A, Das S, Basu S, Kobayashi Y, Kobayashi Y, Koyama H, Ganesan M.
|
| Title |
GhSTOP1, a C2H2 type zinc finger transcription factor is essential for aluminum and proton stress tolerance and lateral root initiation in cotton.
|
| Journal |
Plant Biol (Stuttg)
|
| Abstract |
Aluminum (Al) and proton (H+ ) ions are major acid soil stress factors deleteriously affecting plant root growth and crop yield. In our preliminary studies, cotton (Gossypium hirsutum L.) seedlings displayed very sensitive phenotypes to Al and H+ rhizotoxicities. Based on previous Arabidopsis results, we aimed to characterise the Al stress responsive Sensitive to Proton rhizotoxicity 1 (GhSTOP1) transcription system in cotton using RNAi-mediated down-regulation. With the help of seed embryo apex explants, we developed transgenic cotton plants overexpressing a GhSTOP1-RNAi cassette with NPTII selection. Kanamycin-tolerant T1 seedlings were further considered for Al and H+ stress tolerance studies. Down-regulation of the GhSTOP1 increased sensitivity to Al and proton rhizotoxicities, and root growth was significantly reduced in RNAi lines. The expression profile of GhALMT1 (Aluminum-activated Malate Transporter 1), GhMATE (Multidrug and Toxic Compound Extrusion), GhALS3 (Aluminium Sensitive 3) and key genes involved in the GABA shunt were down-regulated in the transgenic RNAi lines. Additionally, the lateral root initiation process was delayed and expression of GhNAC1, which is involved in lateral roots, was also suppressed in transgenic lines. Besides, overexpression of GhSTOP1 in Arabidopsis accelerated root growth and AtMATE and AtALMT1 expression under Al stress conditions. These analyses indicate that GhSTOP1 is essential for the expression of several genes which are necessary for acid soil tolerance mechanisms and lateral root initiation.
|
| Volume |
21(1)
|
| Pages |
35-44
|
| Published |
2019-1-1
|
| DOI |
10.1111/plb.12895
|
| PMID |
30098101
|
| MeSH |
Adaptation, Physiological / drug effects*
Aluminum / toxicity*
Amino Acid Sequence
Arabidopsis / genetics
Base Sequence
Citrates / metabolism
Cloning, Molecular
Down-Regulation / drug effects
Down-Regulation / genetics
Gene Expression Regulation, Plant / drug effects
Genes, Plant
Gossypium / physiology*
Malates / metabolism
Phenotype
Phylogeny
Plant Proteins / chemistry
Plant Proteins / genetics
Plant Proteins / metabolism*
Plant Roots / drug effects
Plant Roots / physiology*
Plants, Genetically Modified
Protons*
RNA Interference
RNA, Messenger / genetics
RNA, Messenger / metabolism
Soil
Stress, Physiological / drug effects
Transcription Factors / chemistry
Transcription Factors / metabolism*
Zinc Fingers*
|
| IF |
2.393
|
| Times Cited |
6
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
sja05800 |
