Reference - Detail
| RRC ID | 54690 |
|---|---|
| Author | Sato T, Ogawa T. |
| Title | Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method. |
| Journal | Methods Mol Biol |
| Abstract |
Mouse spermatogonial stem cells (SSCs) can be grown in culture for long periods. Cultured SSCs, also called germline stem (GS) cells, maintain themselves by self-renewing proliferation while retaining the ability to differentiate into sperm. Thus, when transplanted into the seminiferous tubules of a host mouse testis, they settle in the basal compartment of the tubules and establish spermatogenenic colonies. The sperm produced in the host are competent to produce offspring. This can be exploited for the generation of genetically modified mice, through the transplantation of genetically modified GS cells. In this section, we describe a method of genome editing-mediated GS cell modification and transplantation. |
| Volume | 1874 |
| Pages | 87-98 |
| Published | 2019-1-1 |
| DOI | 10.1007/978-1-4939-8831-0_5 |
| PMID | 30353509 |
| MeSH | Adult Germline Stem Cells / chemistry Adult Germline Stem Cells / cytology* Animals Cell Culture Techniques Cell Differentiation Cell Proliferation Cells, Cultured Female Gene Editing / methods* Gene Knock-In Techniques Male Mice Mice, Transgenic / genetics Mice, Transgenic / growth & development* RNA, Untranslated / genetics* Rats Spermatogenesis Stem Cell Transplantation |
| Resource | |
| Human and Animal Cells | GS-DG1(AES0002) Mvh-Venus GS(AES0196) |