論文 - 詳細
RRC ID | 5470 |
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著者 | Nishida T, Tsuji S, Tsujii M, Ishii S, Yoshio T, Shinzaki S, Egawa S, Irie T, Kakiuchi Y, Yasumaru M, Iijima H, Tsutsui S, Kawano S, Hayashi N. |
タイトル | Cultured bone marrow cell local implantation accelerates healing of ulcers in mice. |
ジャーナル | J Gastroenterol |
Abstract |
BACKGROUND:The therapeutic potential of bone marrow (BM)-derived cells in ulcers is not known. This study aimed to clarify (1) cell types that are derived from the BM which infiltrate ulcers; (2) whether BM-derived cells or gastric myofibroblasts can be used for cell transplantation to treat ulcers; and (3) the phenotypes of such transplantable cells. METHODS:(1) Wild-type mice were transplanted with BM cells of green fluorescent protein (GFP)-transgenic mice. Acetic acid-induced gastric ulcers were produced in mice after BM transplantation. (2) BM cells and gastric myofibroblasts were isolated from GFP-transgenic mice. Bone marrow cells attached to plastic dishes were selected for expansion. Gastric ulcers were induced, and BM-derived cells, myofibroblasts, or phosphate-buffered saline were injected around ulcers. The ulcer healing process was examined macroscopically and histologically. (3) Expression of growth factors and cytokines in transplantable cells was examined by reverse transcriptase-polymerase chain reaction. RESULTS:(1) GFP-positive cells with interstitial phenotypes were observed at the ulcerated area. (2) Ulcer healing was significantly promoted by the injection of BM-derived cells compared to controls on day 7, but not on day 3. The BM-derived cells were observed in the tissue surrounding the ulcer. However, myofibroblasts were not found. (3) The BM-derived cells expressed hepatocyte growth factor, transforming growth factor-beta(1), and other stromal factors before transplantation, and had mesenchymal phenotypes after transplantation. CONCLUSIONS:BM-derived cells are involved in the ulcer healing. BM-derived cells, but not myofibroblasts, are locally implantable to ulcers. Thus, BM-derived cells can be transplanted to accelerate ulcer healing. |
巻・号 | 43(2) |
ページ | 124-35 |
公開日 | 2008-1-1 |
DOI | 10.1007/s00535-007-2137-6 |
PMID | 18306986 |
MeSH | Acetic Acid / adverse effects Animals Bone Marrow Transplantation* Cell Transdifferentiation Cells, Cultured Female Male Mice Mice, Transgenic Phenotype Stomach Ulcer / chemically induced Stomach Ulcer / physiopathology* Stomach Ulcer / surgery* Wound Healing / physiology* |
IF | 6.132 |
引用数 | 11 |
WOS 分野 | GASTROENTEROLOGY & HEPATOLOGY |
リソース情報 | |
実験動物マウス | RBRC00267 |