論文 - 詳細
| RRC ID | 54731 |
|---|---|
| 著者 | Calistri NL, Kimmerling RJ, Malinowski SW, Touat M, Stevens MM, Olcum S, Ligon KL, Manalis SR. |
| タイトル | Microfluidic active loading of single cells enables analysis of complex clinical specimens. |
| ジャーナル | Nat Commun |
| Abstract |
A fundamental trade-off between flow rate and measurement precision limits performance of many single-cell detection strategies, especially for applications that require biophysical measurements from living cells within complex and low-input samples. To address this, we introduce 'active loading', an automated, optically-triggered fluidic system that improves measurement throughput and robustness by controlling entry of individual cells into a measurement channel. We apply active loading to samples over a range of concentrations (1-1000 particles μL-1), demonstrate that measurement time can be decreased by up to 20-fold, and show theoretically that performance of some types of existing single-cell microfluidic devices can be improved by implementing active loading. Finally, we demonstrate how active loading improves clinical feasibility for acute, single-cell drug sensitivity measurements by deploying it to a preclinical setting where we assess patient samples from normal brain, primary and metastatic brain cancers containing a complex, difficult-to-measure mixture of confounding biological debris. |
| 巻・号 | 9(1) |
| ページ | 4784 |
| 公開日 | 2018-11-14 |
| DOI | 10.1038/s41467-018-07283-x |
| PII | 10.1038/s41467-018-07283-x |
| PMID | 30429479 |
| PMC | PMC6235965 |
| MeSH | Animals Cell Line Cells, Cultured Equipment Design Humans Mice Microfluidic Analytical Techniques / instrumentation* Microfluidic Analytical Techniques / methods* Reproducibility of Results Single-Cell Analysis / instrumentation* Single-Cell Analysis / methods* Tumor Cells, Cultured |
| IF | 11.878 |
| 引用数 | 7 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 22 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Ba/F3(RCB0805) |