| RRC ID |
54750
|
| 著者 |
Chen X, Lei F, Wang L, Xiong X, Song J.
|
| タイトル |
Generation of Tumor Antigen-Specific Cytotoxic T Lymphocytes from Pluripotent Stem Cells.
|
| ジャーナル |
Methods Mol Biol
|
| Abstract |
Immunotherapy is a developing but very promising arsenal to treat cancer. Acquiring a more potent and effective approach in cancer immunotherapy is always the ultimate pursuance. CTL-based therapies are highly acclaimed recently due to its direct killing property. However, difficulty in obtaining adequate number of CTLs is still a major obstacle. In previous studies, it is shown that pluripotent stem cell-derived cytotoxic T lymphocytes (CTL)-especially the genetically engineered tumor antigen-specific CTLs-may serve as a good candidate for this goal. Here we introduce a novel approach in generating tumor antigen-specific CTLs from induced pluripotent stem cells (iPSCs) by using both in vitro and in vivo priming mechanisms for the tumor management in a murine melanoma model.
|
| 巻・号 |
1884
|
| ページ |
43-55
|
| 公開日 |
2019-1-1
|
| DOI |
10.1007/978-1-4939-8885-3_3
|
| PMID |
30465194
|
| PMC |
PMC6629431
|
| MeSH |
Adaptor Proteins, Signal Transducing
Animals
Antigens, Neoplasm / immunology*
Antigens, Neoplasm / metabolism
Calcium-Binding Proteins
Cell Culture Techniques / instrumentation
Cell Culture Techniques / methods*
Cell Differentiation / immunology
Cell Line, Tumor
Female
Immunotherapy, Adoptive / methods*
Induced Pluripotent Stem Cells / physiology
Intercellular Signaling Peptides and Proteins / genetics
Intercellular Signaling Peptides and Proteins / immunology
Intercellular Signaling Peptides and Proteins / metabolism
Intracellular Signaling Peptides and Proteins / genetics
Intracellular Signaling Peptides and Proteins / immunology
Intracellular Signaling Peptides and Proteins / metabolism
Intramolecular Oxidoreductases / immunology
Intramolecular Oxidoreductases / metabolism
Melanoma, Experimental / immunology
Melanoma, Experimental / pathology
Melanoma, Experimental / therapy
Membrane Proteins / genetics
Membrane Proteins / immunology
Membrane Proteins / metabolism
Mice
Mice, Inbred C57BL
Receptors, Antigen, T-Cell / immunology
Receptors, Antigen, T-Cell / metabolism
Skin Neoplasms / immunology
Skin Neoplasms / pathology
Skin Neoplasms / therapy
T-Lymphocytes, Cytotoxic / immunology*
T-Lymphocytes, Cytotoxic / metabolism
T-Lymphocytes, Cytotoxic / transplantation
|
| リソース情報 |
| ヒト・動物細胞 |
iPS-MEF-Ng-20D-17(APS0001) |
