RRC ID 54750
Author Chen X, Lei F, Wang L, Xiong X, Song J.
Title Generation of Tumor Antigen-Specific Cytotoxic T Lymphocytes from Pluripotent Stem Cells.
Journal Methods Mol Biol
Abstract Immunotherapy is a developing but very promising arsenal to treat cancer. Acquiring a more potent and effective approach in cancer immunotherapy is always the ultimate pursuance. CTL-based therapies are highly acclaimed recently due to its direct killing property. However, difficulty in obtaining adequate number of CTLs is still a major obstacle. In previous studies, it is shown that pluripotent stem cell-derived cytotoxic T lymphocytes (CTL)-especially the genetically engineered tumor antigen-specific CTLs-may serve as a good candidate for this goal. Here we introduce a novel approach in generating tumor antigen-specific CTLs from induced pluripotent stem cells (iPSCs) by using both in vitro and in vivo priming mechanisms for the tumor management in a murine melanoma model.
Volume 1884
Pages 43-55
Published 2019-1-1
DOI 10.1007/978-1-4939-8885-3_3
PMID 30465194
PMC PMC6629431
MeSH Adaptor Proteins, Signal Transducing Animals Antigens, Neoplasm / immunology* Antigens, Neoplasm / metabolism Calcium-Binding Proteins Cell Culture Techniques / instrumentation Cell Culture Techniques / methods* Cell Differentiation / immunology Cell Line, Tumor Female Immunotherapy, Adoptive / methods* Induced Pluripotent Stem Cells / physiology Intercellular Signaling Peptides and Proteins / genetics Intercellular Signaling Peptides and Proteins / immunology Intercellular Signaling Peptides and Proteins / metabolism Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / immunology Intracellular Signaling Peptides and Proteins / metabolism Intramolecular Oxidoreductases / immunology Intramolecular Oxidoreductases / metabolism Melanoma, Experimental / immunology Melanoma, Experimental / pathology Melanoma, Experimental / therapy Membrane Proteins / genetics Membrane Proteins / immunology Membrane Proteins / metabolism Mice Mice, Inbred C57BL Receptors, Antigen, T-Cell / immunology Receptors, Antigen, T-Cell / metabolism Skin Neoplasms / immunology Skin Neoplasms / pathology Skin Neoplasms / therapy T-Lymphocytes, Cytotoxic / immunology* T-Lymphocytes, Cytotoxic / metabolism T-Lymphocytes, Cytotoxic / transplantation
Human and Animal Cells iPS-MEF-Ng-20D-17(APS0001)