論文 - 詳細
| RRC ID | 54796 |
|---|---|
| 著者 | Shimo T, Koyama E, Okui T, Masui M, Kunisada Y, Ibaragi S, Yoshioka N, Kurio N, Yoshida S, Sasaki A, Iwamoto M. |
| タイトル | Retinoic Receptor Signaling Regulates Hypertrophic Chondrocyte-specific Gene Expression. |
| ジャーナル | In Vivo |
| Abstract |
BACKGROUND/AIM:Retinoid signaling is important for the maturation of growth-plate chondrocytes. The effect of retinoid receptor gamma (RARγ) signaling on the expression of genes in hypertrophic chondrocytes is unclear. This study investigated the role of RARγ signaling in regulation of hypertrophic chondrocyte-specific genes. MATERIALS AND METHODS:The gene expression in mouse E17.5 tibial cartilage was examined by in situ hybridization analysis. Real-time reverse transcription-polymerase chain reaction (RT-PCR) and immunoblotting were used for analysis of mRNA and phosphorylated mitogen-activated protein kinase (MAPK). RESULTS:mRNA expression of Rarg and connective tissue growth factor (Ccn2) was detected in maturing chondrocytes throughout the cartilaginous skeletal elements. In chondrogenic ATDC5 cells, an RARγ agonist induced the gene expression of type-X collagen (Col10A1), transglutaminase-2 (Tg2), matrix metalloproteinase-13 (Mmp13), and Ccn2 mRNA, whereas a retinoic acid pan-agonist suppressed RARγ agonist-stimulated gene expression. Phosphorylated extracellular signal regulated-kinases (pERK1/2), p-p38, and phosphorylated c-Jun N-terminal kinase (pJNK) MAPK were time-dependently increased by RARγ agonist treatment. Experimental p38 inhibition led to a severe drop in the RARγ agonist-stimulated expressions of Col10A1, Tg2, Mmp13, and Ccn2 mRNA. CONCLUSION:RARγ signaling is required for the differentiation of hypertrophic chondrocytes, with differential cooperation with p38 MAPK. |
| 巻・号 | 33(1) |
| ページ | 85-91 |
| 公開日 | 2019-1-1 |
| DOI | 10.21873/invivo.11443 |
| PII | 33/1/85 |
| PMID | 30587607 |
| PMC | PMC6364088 |
| MeSH | Animals Cartilage / growth & development Cartilage / metabolism Cell Differentiation / genetics* Chondrocytes / cytology Chondrocytes / metabolism* Collagen Type X / genetics Connective Tissue Growth Factor / genetics Gene Expression Regulation, Developmental / genetics Humans In Situ Hybridization Matrix Metalloproteinase 13 / genetics Mice Phosphorylation RNA, Messenger / genetics Receptors, Retinoic Acid / genetics* Signal Transduction / genetics Tibia / growth & development Tibia / metabolism Tretinoin / metabolism* p38 Mitogen-Activated Protein Kinases / genetics |
| IF | 1.609 |
| 引用数 | 3 |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) |