論文 - 詳細
| RRC ID | 54797 |
|---|---|
| 著者 | Shimizu D, Masuda T, Sato K, Tsuruda Y, Otsu H, Kuroda Y, Eguchi H, Kodera Y, Mimori K. |
| タイトル | CRMP5-associated GTPase (CRAG) Is a Candidate Driver Gene for Colorectal Cancer Carcinogenesis. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND/AIM:Certain chromosomal arms are clonally amplified in colorectal cancer (CRC) and may contain novel driver genes. The aim of this study was to identify a novel driver gene for colorectal cancer carcinogenesis on long arm of chromosome 7 and the clarify its biological function. MATERIALS AND METHODS:We identified ArfGAP with GTPase domain, ankyrin repeat and PH domain 3 (AGAP3) as a putative driver gene using the CRC dataset in The Cancer Genome Atlas (TCGA). Biological functions of AGAP3 and CRMP5-associated GTPase (CRAG), a splicing variant of AGAP3, were explored by overexpression. AGAP3/CRAG expression in our cohort was examined by quantitative reverse transcription polymerase chain reaction. Clinical significance of AGAP3/CRAG expression in TCGA dataset, Gene Expression Omnibus datasets and our clinical cohort was evaluated. RESULTS:AGAP3 expression was significantly increased in CRC and colorectal adenoma compared to normal tissue. CRAG overexpression up-regulated c-Jun expression, and significantly increased cell proliferation and colony formation capability. AGAP3 expression did not have a concordant association with patient prognosis among datasets. CONCLUSION:CRAG may contribute to development of CRC via activator protein 1 activation. |
| 巻・号 | 39(1) |
| ページ | 99-106 |
| 公開日 | 2019-1-1 |
| DOI | 10.21873/anticanres.13084 |
| PII | 39/1/99 |
| PMID | 30591445 |
| MeSH | Adenoma / genetics* Adenoma / pathology Aged Carcinogenesis / genetics* Cell Line, Tumor Cell Proliferation / genetics Colorectal Neoplasms / genetics* Colorectal Neoplasms / pathology Female GTP Phosphohydrolases / genetics* Gene Expression Regulation, Neoplastic / genetics Humans Hydrolases Male Microtubule-Associated Proteins Middle Aged Nerve Tissue Proteins / genetics Prognosis |
| IF | 1.935 |
| 引用数 | 1 |
| リソース情報 | |
| ヒト・動物細胞 | HCT116(RCB2979) |