RRC ID 54848
著者 Zhang W, Matsuo R, Takahara T, Shibata H, Maki M.
タイトル High Sensitive Quantitative Binding Assays Using a Nanoluciferase-Fused Probe for Analysis of ALG-2-Interacting Proteins.
ジャーナル Methods Mol Biol
Abstract Many non-catalytic cellular proteins exert biological functions by formation of stable or transient complexes with other proteins. Analysis of the signal-induced physical interactions is important to understand their physiological roles in cells. Here we describe a biochemical method for assessing the binding of ALG-2 (gene name, PDCD6) to its target proteins that are immunoprecipitated from cell lysates. Application of nanoluciferase (Nluc)-fused ALG-2 enables a rapid quantitative evaluation of Ca2+-dependent interactions of target proteins with ALG-2 in vitro binding assays.
巻・号 1929
ページ 501-516
公開日 2019-1-1
DOI 10.1007/978-1-4939-9030-6_31
PMID 30710293
MeSH Apoptosis Regulatory Proteins / genetics* Apoptosis Regulatory Proteins / metabolism* Calcium / metabolism* Calcium-Binding Proteins / genetics* Calcium-Binding Proteins / metabolism* Escherichia coli / genetics Escherichia coli / growth & development HEK293 Cells Humans Immunoprecipitation Luciferases / genetics* Nanotechnology Protein Binding Recombinant Fusion Proteins / metabolism
リソース情報
ヒト・動物細胞 293(RCB1637)