論文 - 詳細
RRC ID | 54848 |
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著者 | Zhang W, Matsuo R, Takahara T, Shibata H, Maki M. |
タイトル | High Sensitive Quantitative Binding Assays Using a Nanoluciferase-Fused Probe for Analysis of ALG-2-Interacting Proteins. |
ジャーナル | Methods Mol Biol |
Abstract |
Many non-catalytic cellular proteins exert biological functions by formation of stable or transient complexes with other proteins. Analysis of the signal-induced physical interactions is important to understand their physiological roles in cells. Here we describe a biochemical method for assessing the binding of ALG-2 (gene name, PDCD6) to its target proteins that are immunoprecipitated from cell lysates. Application of nanoluciferase (Nluc)-fused ALG-2 enables a rapid quantitative evaluation of Ca2+-dependent interactions of target proteins with ALG-2 in vitro binding assays. |
巻・号 | 1929 |
ページ | 501-516 |
公開日 | 2019-1-1 |
DOI | 10.1007/978-1-4939-9030-6_31 |
PMID | 30710293 |
MeSH | Apoptosis Regulatory Proteins / genetics* Apoptosis Regulatory Proteins / metabolism* Calcium / metabolism* Calcium-Binding Proteins / genetics* Calcium-Binding Proteins / metabolism* Escherichia coli / genetics Escherichia coli / growth & development HEK293 Cells Humans Immunoprecipitation Luciferases / genetics* Nanotechnology Protein Binding Recombinant Fusion Proteins / metabolism |
リソース情報 | |
ヒト・動物細胞 | 293(RCB1637) |