論文 - 詳細
RRC ID | 54852 |
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著者 | Shinada H, Watanabe T, Okudaira K, Iwase Y, Nishi K, Yumita N. |
タイトル | Apoptosis Induced by Ultraviolet A Exposure in the Presence of Enoxacin in HL-60 Cells. |
ジャーナル | Anticancer Res |
Abstract |
BACKGROUND:The ultraviolet A (UVA) spectrum mainly includes the region associated with the phototoxicity of fluoroquinolone antimicrobial agents. This study investigated apoptosis induced with UVA light and enoxacin in HL-60 cells. MATERIALS AND METHODS:HL-60 cells were irradiated by UVA (1.1 mW/cm2) for 20 min in the presence or absence of enoxacin. The induction of apoptosis was investigated by analysing cell morphology, flow cytometry of annexin V-positive cells, DNA ladder formation, and caspase-3 activation. RESULTS:Significant induction of apoptosis, DNA fragmentation, and caspase-3 activation were observed in cells treated with both UVA and enoxacin. UVA-induced apoptosis was significantly suppressed when NaN3, a singlet oxygen scavenger, was present. CONCLUSION:Apoptosis was induced by the combination of UVA and enoxacin in HL-60 cells, and singlet oxygen appears to play an important role in photodynamically-induced apoptosis. |
巻・号 | 39(2) |
ページ | 687-693 |
公開日 | 2019-2-1 |
DOI | 10.21873/anticanres.13164 |
PII | 39/2/687 |
PMID | 30711946 |
MeSH | Apoptosis* Caspase 3 / metabolism DNA / drug effects* DNA / radiation effects* DNA Fragmentation / drug effects Enoxacin / pharmacology* Flow Cytometry Fluoroquinolones / pharmacology Free Radical Scavengers HL-60 Cells Humans Photosensitizing Agents / chemistry Reactive Oxygen Species Sodium Azide / chemistry Ultraviolet Rays* |
IF | 1.935 |
引用数 | 1 |
リソース情報 | |
ヒト・動物細胞 | HL-60(RCB3683) |