RRC ID 54852
Author Shinada H, Watanabe T, Okudaira K, Iwase Y, Nishi K, Yumita N.
Title Apoptosis Induced by Ultraviolet A Exposure in the Presence of Enoxacin in HL-60 Cells.
Journal Anticancer Res
Abstract BACKGROUND:The ultraviolet A (UVA) spectrum mainly includes the region associated with the phototoxicity of fluoroquinolone antimicrobial agents. This study investigated apoptosis induced with UVA light and enoxacin in HL-60 cells.
MATERIALS AND METHODS:HL-60 cells were irradiated by UVA (1.1 mW/cm2) for 20 min in the presence or absence of enoxacin. The induction of apoptosis was investigated by analysing cell morphology, flow cytometry of annexin V-positive cells, DNA ladder formation, and caspase-3 activation.
RESULTS:Significant induction of apoptosis, DNA fragmentation, and caspase-3 activation were observed in cells treated with both UVA and enoxacin. UVA-induced apoptosis was significantly suppressed when NaN3, a singlet oxygen scavenger, was present.
CONCLUSION:Apoptosis was induced by the combination of UVA and enoxacin in HL-60 cells, and singlet oxygen appears to play an important role in photodynamically-induced apoptosis.
Volume 39(2)
Pages 687-693
Published 2019-2-1
DOI 10.21873/anticanres.13164
PII 39/2/687
PMID 30711946
MeSH Apoptosis* Caspase 3 / metabolism DNA / drug effects* DNA / radiation effects* DNA Fragmentation / drug effects Enoxacin / pharmacology* Flow Cytometry Fluoroquinolones / pharmacology Free Radical Scavengers HL-60 Cells Humans Photosensitizing Agents / chemistry Reactive Oxygen Species Sodium Azide / chemistry Ultraviolet Rays*
IF 1.935
Times Cited 1
Human and Animal Cells HL-60(RCB3683)