RRC ID 54857
Author Ono R, Yasuhiko Y, Aisaki KI, Kitajima S, Kanno J, Hirabayashi Y.
Title Exosome-mediated horizontal gene transfer occurs in double-strand break repair during genome editing.
Journal Commun Biol
Abstract The CRISPR-Cas9 system has been successfully applied in many organisms as a powerful genome-editing tool. Undoubtedly, it will soon be applied to human genome editing, including gene therapy. We have previously reported that unintentional DNA sequences derived from retrotransposons, genomic DNA, mRNA and vectors are captured at double-strand breaks (DSBs) sites when DSBs are introduced by the CRISPR-Cas9 system. Therefore, it is possible that unintentional insertions associated with DSB repair represent a potential risk for human genome editing gene therapies. To address this possibility, comprehensive sequencing of DSB sites was performed. Here, we report that exosome-mediated horizontal gene transfer occurs in DSB repair during genome editing. Exosomes are present in all fluids from living animals, including seawater and breathing mammals, suggesting that exosome-mediated horizontal gene transfer is the driving force behind mammalian genome evolution. The findings of this study highlight an emerging new risk for this leading-edge technology.
Volume 2
Pages 57
Published 2019-2-8
DOI 10.1038/s42003-019-0300-2
PII 300
PMID 30775458
PMC PMC6368560
MeSH Animals CRISPR-Cas Systems* Cattle DNA / genetics* DNA / metabolism DNA Breaks, Double-Stranded DNA Repair* Embryo, Mammalian Escherichia coli / genetics Escherichia coli / metabolism Exosomes / genetics* Exosomes / metabolism Gene Editing / ethics* Gene Transfer, Horizontal* Genome* Goats High-Throughput Nucleotide Sequencing Humans Mice NIH 3T3 Cells Plasmids / chemistry Plasmids / metabolism Retroelements Short Interspersed Nucleotide Elements
IF 4.165
Human and Animal Cells NIH/3T3(RCB2767)