| RRC ID |
54865
|
| 著者 |
Kang JH, Miettinen TP, Chen L, Olcum S, Katsikis G, Doyle PS, Manalis SR.
|
| タイトル |
Noninvasive monitoring of single-cell mechanics by acoustic scattering.
|
| ジャーナル |
Nat Methods
|
| Abstract |
The monitoring of mechanics in a single cell throughout the cell cycle has been hampered by the invasiveness of mechanical measurements. Here we quantify mechanical properties via acoustic scattering of waves from a cell inside a fluid-filled vibrating cantilever with a temporal resolution of < 1 min. Through simulations, experiments with hydrogels and the use of chemically perturbed cells, we show that our readout, the size-normalized acoustic scattering (SNACS), measures stiffness. To demonstrate the noninvasiveness of SNACS over successive cell cycles, we used measurements that resulted in deformations of < 15 nm. The cells maintained constant SNACS throughout interphase but showed dynamic changes during mitosis. Our work provides a basis for understanding how growing cells maintain mechanical integrity, and demonstrates that acoustic scattering can be used to noninvasively probe subtle and transient dynamics.
|
| 巻・号 |
16(3)
|
| ページ |
263-269
|
| 公開日 |
2019-3-1
|
| DOI |
10.1038/s41592-019-0326-x
|
| PII |
10.1038/s41592-019-0326-x
|
| PMID |
30742041
|
| PMC |
PMC6420125
|
| MeSH |
Acoustics*
Animals
Biomechanical Phenomena
Cell Cycle
Dactinomycin / metabolism
Mice
Microfluidics
Single-Cell Analysis / methods*
|
| IF |
28.467
|
| 引用数 |
9
|
| リソース情報 |
| ヒト・動物細胞 |
Ba/F3(RCB0805) |
