論文 - 詳細
| RRC ID | 54865 |
|---|---|
| 著者 | Kang JH, Miettinen TP, Chen L, Olcum S, Katsikis G, Doyle PS, Manalis SR. |
| タイトル | Noninvasive monitoring of single-cell mechanics by acoustic scattering. |
| ジャーナル | Nat Methods |
| Abstract |
The monitoring of mechanics in a single cell throughout the cell cycle has been hampered by the invasiveness of mechanical measurements. Here we quantify mechanical properties via acoustic scattering of waves from a cell inside a fluid-filled vibrating cantilever with a temporal resolution of < 1 min. Through simulations, experiments with hydrogels and the use of chemically perturbed cells, we show that our readout, the size-normalized acoustic scattering (SNACS), measures stiffness. To demonstrate the noninvasiveness of SNACS over successive cell cycles, we used measurements that resulted in deformations of < 15 nm. The cells maintained constant SNACS throughout interphase but showed dynamic changes during mitosis. Our work provides a basis for understanding how growing cells maintain mechanical integrity, and demonstrates that acoustic scattering can be used to noninvasively probe subtle and transient dynamics. |
| 巻・号 | 16(3) |
| ページ | 263-269 |
| 公開日 | 2019-3-1 |
| DOI | 10.1038/s41592-019-0326-x |
| PII | 10.1038/s41592-019-0326-x |
| PMID | 30742041 |
| PMC | PMC6420125 |
| MeSH | Acoustics* Animals Biomechanical Phenomena Cell Cycle Dactinomycin / metabolism Mice Microfluidics Single-Cell Analysis / methods* |
| IF | 28.467 |
| 引用数 | 9 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 30 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Ba/F3(RCB0805) |