| RRC ID |
54872
|
| 著者 |
Hayakawa K, Kawasaki M, Hirai T, Yoshida Y, Tsushima H, Fujishiro M, Ikeda K, Morimoto S, Takamori K, Sekigawa I.
|
| タイトル |
MicroRNA-766-3p Contributes to Anti-Inflammatory Responses through the Indirect Inhibition of NF-κB Signaling.
|
| ジャーナル |
Int J Mol Sci
|
| Abstract |
MicroRNA (miRNA) is small RNA of 20 to 22 nucleotides in length and is stably present in plasma. Regulating the expression of miRNA taken into cells has been suggested as a general therapeutic approach. We identified the novel anti-inflammatory miRNA hsa-miR-766-3p and investigated its biological function in human rheumatoid arthritis (RA) fibroblast-like synoviocyte MH7A cells. To verify the function of the miRNA present in the plasma of RA patients, we performed a comprehensive analysis of the miRNA expression during abatacept treatment and identified eight miRNAs with significantly altered expression levels. Among these eight miRNAs, miR-766-3p was found to have a clear function. The expression of inflammatory genes in response to inflammatory stimuli was suppressed in MH7A transduced with miR-766-3p. We showed that miR-766-3p indirectly reduced the activation of NF-κB and clarified that this mechanism was partially involved in the reduction of the mineralocorticoid receptor expression. In addition, the inflammatory responses were suppressed in other types of cells. These results indicate the novel function of miR-766-3p, findings that may aid in the development of therapies to suppress inflammation, not only in RA but also in other diseases.
|
| 巻・号 |
20(4)
|
| 公開日 |
2019-2-14
|
| DOI |
10.3390/ijms20040809
|
| PII |
ijms20040809
|
| PMID |
30769772
|
| PMC |
PMC6413049
|
| MeSH |
Abatacept / administration & dosage
Anti-Inflammatory Agents / administration & dosage
Arthritis, Rheumatoid / blood
Arthritis, Rheumatoid / drug therapy*
Arthritis, Rheumatoid / genetics*
Arthritis, Rheumatoid / pathology
Gene Expression Regulation / drug effects
Humans
Inflammation / blood
Inflammation / drug therapy
Inflammation / genetics
Inflammation / pathology
MicroRNAs / genetics*
NF-kappa B / genetics
Receptors, Mineralocorticoid / genetics*
Signal Transduction / drug effects
Synoviocytes / drug effects
Synoviocytes / pathology
|
| IF |
4.183
|
| 引用数 |
3
|
| リソース情報 |
| ヒト・動物細胞 |
MH7A(RCB1512) |
