RRC ID 54946
Author Slane D, Reichardt I, El Kasmi F, Bayer M, Jürgens G.
Title Evolutionarily diverse SYP1 Qa-SNAREs jointly sustain pollen tube growth in Arabidopsis.
Journal Plant J
Abstract Intracellular membrane fusion is effected by SNARE proteins that reside on adjacent membranes and form bridging trans-SNARE complexes. Qa-SNARE members of the Arabidopsis SYP1 family are involved in membrane fusion at the plasma membrane or during cell plate formation. Three SYP1 family members have been classified as pollen-specific as inferred from gene expression profiling studies, and two of them, SYP124 and SYP125, are confined to angiosperms. The SYP124 gene appears genetically unstable, whereas its sister gene SYP125 shows essentially no variation among Arabidopsis accessions. The third pollen-specific member SYP131 is sister to SYP132, which appears evolutionarily conserved in the plant lineage. Although evolutionarily diverse, the three SYP1 proteins are functionally overlapping in that only the triple mutant syp124 syp125 syp131 shows a specific and severe male gametophytic defect. While pollen development and germination appear normal, pollen tube growth is arrested during passage through the style. Our results suggest that angiosperm pollen tubes employ a combination of ancient and modern Qa-SNARE proteins to sustain their growth-promoting membrane dynamics during the reproductive process.
Volume 92(3)
Pages 375-385
Published 2017-11
DOI 10.1111/tpj.13659
PMID 28792633
MeSH Arabidopsis / cytology Arabidopsis / genetics* Arabidopsis / growth & development Arabidopsis / metabolism Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Biological Evolution Cell Membrane / metabolism Cell Proliferation Gene Expression Profiling Gene Expression Regulation, Plant* Organ Specificity Phylogeny Pollination Protein Transport Qa-SNARE Proteins / genetics Qa-SNARE Proteins / metabolism*
IF 5.726
Times Cited 5
Resource
Arabidopsis / Cultured plant cells, genes pst11980