RRC ID 54954
Author Yang Y, Chou HL, Crofts AJ, Zhang L, Tian L, Washida H, Fukuda M, Kumamaru T, Oviedo OJ, Starkenburg SR, Okita TW.
Title Selective sets of mRNAs localize to extracellular paramural bodies in a rice glup6 mutant.
Journal J Exp Bot
Abstract The transport of rice glutelin storage proteins to the storage vacuoles requires the Rab5 GTPase and its related guanine nucleotide exchange factor (Rab5-GEF). Loss of function of these membrane vesicular trafficking factors results in the initial secretion of storage proteins and later their partial engulfment by the plasma membrane to form an extracellular paramural body (PMB), an aborted endosome complex. Here, we show that in the rice Rab5-GEF mutant glup6, glutelin RNAs are specifically mislocalized from their normal location on the cisternal endoplasmic reticulum (ER) to the protein body-ER, and are also apparently translocated to the PMBs. We substantiated the association of mRNAs with this aborted endosome complex by RNA-seq of PMBs purified by flow cytometry. Two PMB-associated groups of RNA were readily resolved: those that were specifically enriched in this aborted complex and those that were highly expressed in the cytoplasm. Examination of the PMB-enriched RNAs indicated that they were not a random sampling of the glup6 transcriptome but, instead, encompassed only a few functional mRNA classes. Although specific autophagy is also an alternative mechanism, our results support the view that RNA localization may co-opt membrane vesicular trafficking, and that many RNAs that share function or intracellular location are co-transported in developing rice seeds.
Volume 69(21)
Pages 5045-5058
Published 2018-10-12
DOI 10.1093/jxb/ery297
PII 5068375
PMID 30102323
PMC PMC6189835
MeSH Glutens / genetics* Glutens / metabolism Guanine Nucleotide Exchange Factors / genetics* Guanine Nucleotide Exchange Factors / metabolism Oryza / genetics* Oryza / metabolism Plant Proteins / genetics* Plant Proteins / metabolism RNA, Messenger / genetics* RNA, Messenger / metabolism RNA, Plant / genetics* RNA, Plant / metabolism rab5 GTP-Binding Proteins / genetics* rab5 GTP-Binding Proteins / metabolism
IF 5.36
Times Cited 5
Rice Induced mutation lines