RRC ID 54971
著者 Sun T, Song Y, Dai J, Mao D, Ma M, Ni JQ, Liang X, Pastor-Pareja JC.
タイトル Spectraplakin Shot Maintains Perinuclear Microtubule Organization in Drosophila Polyploid Cells.
ジャーナル Dev Cell
Abstract Polyploid cells endoreplicate their DNA through a modified cell cycle that skips mitosis as part of their differentiation programs. Upon cell-cycle exit and differentiation, non-centrosomal sites govern microtubule distribution in most cells. Little is known on how polyploid cells, differentiated but cycling, organize their microtubules. We show that microtubules in Drosophila adipocytes and other polyploid tissues form a dense perinuclear cortex responsible for nuclear size and position. Confirming a relation between this perinuclear cortex and the polyploid endocycle, polyploidization of normally diploid cells was sufficient for cortex formation. A critical component of the perinuclear microtubule organizer (pnMTOC) is Shot, absence of which caused collapse of the perinuclear network into a condensed organizer through kinesin-dependent microtubule sliding. Furthermore, this ectopic organizer was capable of directing partial assembly of a deeply disruptive cytokinesis furrow. In all, our study revealed the importance of perinuclear microtubule organization for stability of endocycling Drosophila cells.
巻・号 49(5)
ページ 731-747.e7
公開日 2019-6-3
DOI 10.1016/j.devcel.2019.03.027
PII S1534-5807(19)30238-2
PMID 31006649
MeSH Animals Cell Nucleus / genetics Cell Nucleus / metabolism Cytokinesis Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / physiology* Female Katanin / genetics Katanin / metabolism* Male Microfilament Proteins / genetics Microfilament Proteins / metabolism* Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism* Microtubules / physiology* Polyploidy* Spindle Apparatus
IF 9.19
引用数 1
リソース情報
ショウジョウバエ 9359R-2 18102R-2 DGRC#110692 DGRC#110807 DGRC#112001 DGRC#130610 DGRC#109838 DGRC#109822