RRC ID 54996
Author Selland LG, Koch S, Laraque M, Waskiewicz AJ.
Title Coordinate regulation of retinoic acid synthesis by pbx genes and fibroblast growth factor signaling by hoxb1b is required for hindbrain patterning and development.
Journal Mech Dev
Abstract The vertebrate hindbrain is composed of a series of lineage-restricted segments termed rhombomeres. Segment-specific gene expression drives unique programs of neuronal differentiation. Two critical embryonic signaling pathways, Fibroblast Growth Factor (FGF) and Retinoic Acid (RA), regulate early embryonic rhombomere patterning. The earliest expressed hox genes, hoxb1b and hoxb1a in zebrafish, are logical candidates for establishing signaling networks that specify segmental identity. We sought to determine the mechanism by which hox genes regulate hindbrain patterning in zebrafish. We demonstrate that hoxb1a regulates r4-specific patterning, while hoxb1b regulates rhombomere segmentation and size. Hoxb1a and hoxb1b redundantly regulate vhnf1 expression. Loss of hoxb1b together with pbx4 reverts the hindbrain to a groundstate identity, demonstrating the importance of hox genes in patterning nearly the entire hindbrain, and a key requirement for Pbx in this process. Additionally, we provide evidence that while pbx genes regulate RA signaling, hoxb1b regulates hindbrain identity through complex regulation of FGF signaling.
Volume 150
Pages 28-41
Published 2018-4-1
DOI 10.1016/j.mod.2018.02.005
PII S0925-4773(17)30784-0
PMID 29496480
MeSH Animals Body Patterning / genetics Embryo, Nonmammalian Embryonic Development / genetics Fibroblast Growth Factors / genetics* Gene Expression Regulation, Developmental / genetics Homeodomain Proteins / genetics* In Situ Hybridization Neurons / metabolism Pre-B-Cell Leukemia Transcription Factor 1 / genetics* Rhombencephalon / growth & development Rhombencephalon / metabolism Signal Transduction / genetics Tretinoin / metabolism* Zebrafish / genetics Zebrafish / growth & development Zebrafish Proteins / genetics*
IF 2.176
Times Cited 2
Zebrafish Tg(CM-isl1:GFP)