RRC ID 55015
Author Takahashi K, Ishii K, Yamashita M.
Title Staufen1, Kinesin1 and microtubule function in cyclin B1 mRNA transport to the animal polar cytoplasm of zebrafish oocytes.
Journal Biochem Biophys Res Commun
Abstract In zebrafish oocytes, cyclin B1 mRNAs are transported to the animal polar cytoplasm. To elucidate the molecular basis of cyclin B1 mRNA transport, we analyzed zebrafish Staufen1, a protein known to play a central role in mRNA transport to the vegetal pole of Xenopus oocytes. Zebrafish Staufen1 interacts with cyclin B1 mRNA throughout oocyte growth. Both cyclin B1 mRNA and Staufen1 are evenly distributed in the cytoplasm of young oocytes but are co-localized to the animal polar cytoplasm in later stages. Real-time imaging showed that the plus ends of oocyte microtubules are free in the cytoplasm in early stages but anchored to the animal polar cytoplasm in later stages. Transport of cyclin B1 reporter mRNA to the animal polar cytoplasm was inhibited by disruption of microtubules and injection of antibodies against Staufen1 or Kinesin1, a plus-end-directed microtubule motor that interacts with Staufen1, indicating that the transport depends on movement along microtubules toward the plus ends. Reporter mRNAs with an element required for the vegetal localization of vg1 mRNA in Xenopus oocytes were localized to the animal polar cytoplasm in zebrafish oocytes, indicating that the element is functional for animal polar localization in zebrafish oocytes. Our findings suggest that cyclin B1 mRNA-Staufen1 protein complexes are transported toward the animal pole of zebrafish oocytes by the plus-end-directed motor protein Kinesin1 along microtubules and that a common mRNA transport machinery functions in zebrafish and Xenopus oocytes, although its transport direction is opposite due to different organizations of microtubules.
Volume 503(4)
Pages 2778-2783
Published 2018-9-18
DOI 10.1016/j.bbrc.2018.08.039
PII S0006-291X(18)31719-4
PMID 30103945
MeSH Animals Antibodies, Neutralizing / pharmacology Biological Transport Cell Polarity / genetics Cyclin B1 / antagonists & inhibitors Cyclin B1 / genetics* Cyclin B1 / metabolism Female Gene Expression Regulation, Developmental Kinesins / genetics* Kinesins / metabolism Microtubules / metabolism Microtubules / ultrastructure Oocytes / growth & development Oocytes / metabolism* Oocytes / ultrastructure Oogenesis / genetics RNA, Messenger / genetics RNA, Messenger / metabolism RNA-Binding Proteins / genetics* RNA-Binding Proteins / metabolism Signal Transduction Time-Lapse Imaging Transforming Growth Factor beta / genetics Transforming Growth Factor beta / metabolism Xenopus Proteins / genetics Xenopus Proteins / metabolism Xenopus laevis / genetics* Xenopus laevis / growth & development Xenopus laevis / metabolism Zebrafish / genetics* Zebrafish / growth & development Zebrafish / metabolism Zebrafish Proteins / genetics* Zebrafish Proteins / metabolism
IF 2.705
Times Cited 3
Zebrafish Tg(-0.5zp3b:Mmu.Mapre1-GFP)