RRC ID 55041
Author Chumakov I, Rigault P, Guillou S, Ougen P, Billaut A, Guasconi G, Gervy P, LeGall I, Soularue P, Grinas L.
Title Continuum of overlapping clones spanning the entire human chromosome 21q.
Journal Nature
Abstract A continuous array of overlapping clones covering the entire human chromosome 21q was constructed from human yeast artificial chromosome libraries using sequence-tagged sites as landmarks specifically detected by polymerase chain reaction. The yeast artificial chromosome contiguous unit starts with pericentromeric and ends with subtelomeric loci of 21q. The resulting order of sequence-tagged sites is consistent with other physical and genetic mapping data. This set of overlapping clones will promote our knowledge of the structure of this chromosome and the function of its genes.
Volume 359(6394)
Pages 380-7
Published 1992-10-1
DOI 10.1038/359380a0
PMID 1406950
MeSH Chromosome Mapping / methods* Chromosomes, Fungal Chromosomes, Human, Pair 21* Cloning, Molecular Gene Library Genetic Markers Genome, Human* Humans Polymerase Chain Reaction Sequence Tagged Sites
IF 43.07
Times Cited 481
DNA material CEPH MEGA YAC clone (RDB04215)