RRC ID 55042
Author Sharov AA, Piao Y, Matoba R, Dudekula DB, Qian Y, VanBuren V, Falco G, Martin PR, Stagg CA, Bassey UC, Wang Y, Carter MG, Hamatani T, Aiba K, Akutsu H, Sharova L, Tanaka TS, Kimber WL, Yoshikawa T, Jaradat SA, Pantano S, Nagaraja R, Boheler KR, Taub D, Hodes RJ, Longo DL, Schlessinger D, Keller J, Klotz E, Kelsoe G, Umezawa A, Vescovi AL, Rossant J, Kunath T, Hogan BL, Curci A, D'Urso M, Kelso J, Hide W, Ko MS.
Title Transcriptome analysis of mouse stem cells and early embryos.
Journal PLoS Biol
Abstract Understanding and harnessing cellular potency are fundamental in biology and are also critical to the future therapeutic use of stem cells. Transcriptome analysis of these pluripotent cells is a first step towards such goals. Starting with sources that include oocytes, blastocysts, and embryonic and adult stem cells, we obtained 249,200 high-quality EST sequences and clustered them with public sequences to produce an index of approximately 30,000 total mouse genes that includes 977 previously unidentified genes. Analysis of gene expression levels by EST frequency identifies genes that characterize preimplantation embryos, embryonic stem cells, and adult stem cells, thus providing potential markers as well as clues to the functional features of these cells. Principal component analysis identified a set of 88 genes whose average expression levels decrease from oocytes to blastocysts, stem cells, postimplantation embryos, and finally to newborn tissues. This can be a first step towards a possible definition of a molecular scale of cellular potency. The sequences and cDNA clones recovered in this work provide a comprehensive resource for genes functioning in early mouse embryos and stem cells. The nonrestricted community access to the resource can accelerate a wide range of research, particularly in reproductive and regenerative medicine.
Volume 1(3)
Pages E74
Published 2003-12-1
DOI 10.1371/journal.pbio.0000074
PMID 14691545
PMC PMC300684
MeSH Animals Animals, Newborn Blastocyst / cytology Blastocyst / metabolism Computational Biology DNA, Complementary / metabolism Databases, Genetic Embryo, Mammalian / metabolism* Expressed Sequence Tags Gene Expression Regulation, Developmental* Gene Library Mice Models, Genetic Molecular Sequence Data Oocytes / cytology Oocytes / metabolism Principal Component Analysis RNA, Messenger / metabolism Sequence Analysis, DNA Stem Cells / cytology* Transcription, Genetic*
IF 8.386
Times Cited 145
DNA material NIA/NIH Mouse cDNA Clone (RDB05723)