RRC ID 55123
著者 Kajikawa A, Suzuki S, Igimi S.
タイトル The impact of motility on the localization of Lactobacillus agilis in the murine gastrointestinal tract.
ジャーナル BMC Microbiol
Abstract BACKGROUND:While the overall composition of the mammalian gut microbiota has been intensively studied, the characteristics and ecologies of individual gut species are incompletely understood. Lactobacilli are considered beneficial commensals in the gastrointestinal mucosa and are relatively well-studied except for the uncommon species which exhibit motility. In this study, we evaluate the importance of motility on gut colonization by comparing motile and non-motile strains of Lactobacillus agilis in mice models.
RESULTS:A flagellated but non-motile L. agilis strain was constructed by mutation of the motB gene. Colonization of the wild type and the mutant strain was assessed in both antibiotic-treated female Balb/c mice and gnotobiotic mice. The results suggest that the motile strain is better able to persist and/or localize in the gut mucosa. Chemotaxis assays indicated that the motile L. agilis strain is attracted by mucin, which is a major component of the intestinal mucus layer in animal guts.
CONCLUSIONS:Motility and chemotactic ability likely confer advantages in gut colonization to L. agilis. These findings suggest that the motile lactobacilli have unique ecologies compared to non-motile commensals of the lactic acid bacteria.
巻・号 18(1)
ページ 68
公開日 2018-7-11
DOI 10.1186/s12866-018-1219-3
PII 10.1186/s12866-018-1219-3
PMID 29996774
PMC PMC6042280
MeSH Animals Anti-Bacterial Agents / pharmacology Bacterial Proteins / genetics Chemotaxis / drug effects Colony Count, Microbial Feces / microbiology Female Gastrointestinal Tract / chemistry Gastrointestinal Tract / microbiology* Intestinal Mucosa / chemistry Intestinal Mucosa / microbiology Lactobacillus / drug effects Lactobacillus / genetics Lactobacillus / growth & development Lactobacillus / physiology* Locomotion* / drug effects Locomotion* / genetics Mice, Inbred BALB C Mucins / pharmacology Mutation
IF 3.287
引用数 1
リソース情報
一般微生物 JCM 1048