RRC ID 55164
Author Xia Y, Cao J, Wang M, Lu M, Chen G, Gao F, Liu Z, Zhang D, Ke X, Yi M.
Title Effects of Lactococcus lactis subsp. lactis JCM5805 on colonization dynamics of gut microbiota and regulation of immunity in early ontogenetic stages of tilapia.
Journal Fish Shellfish Immunol.
Abstract . The survival rate of T2 was significantly higher than that of the C group (P < 0.05). Microbial characterization by Illumina HiSeq sequencing of 16S rRNA gene amplicons showed the significantly higher presence of JCM5805 in the guts of T2 after 15 days of probiotic continuous application. Although JCM5805 was below the detection level after the cessation of probiotic for 5 days, the gut microbiota of the exposed tilapia larvae in T2 remained clearly different from that of the control treatment after the cessation of probiotic administration. These data indicated that a high concentration of the probiotic strain JCM5805 upregulated the expression of IFNα via the TLR7/TLR9-Myd88 pathway and enhanced disease resistance of larvae. JCM5805 was only transiently detected and thus was not included in the stable larval microbiota. The early microbial exposure of tilapia larvae affects the gut microbiota at later life stages. However, whether the upregulation of related genes is related to the presence of JCM5805 strain in the intestine requires further verification.
Volume 86
Pages 53-63
Published 2019-3
DOI 10.1016/j.fsi.2018.11.022
PII S1050-4648(18)30741-1
PMID 30428393
MeSH Animals Gastrointestinal Microbiome / immunology* Gene Expression Regulation, Developmental / immunology Lactococcus lactis / physiology* Probiotics Random Allocation Tilapia / growth & development* Tilapia / immunology Tilapia / microbiology* Transcriptome
IF 3.185
Resource
General Microbes JCM 5805