| RRC ID |
55168
|
| Author |
Kawashima T, Ikari N, Watanabe Y, Kubota Y, Yoshio S, Kanto T, Motohashi S, Shimojo N, Tsuji NM.
|
| Title |
Double-Stranded RNA Derived from Lactic Acid Bacteria Augments Th1 Immunity via Interferon-β from Human Dendritic Cells.
|
| Journal |
Front Immunol
|
| Abstract |
Lactic acid bacteria (LAB) are one of the major commensal species in the small intestine and known for contributing to maintenance of protective immunity and immune homeostasis. However, currently there has been no evidence regarding the cellular mechanisms involved in the probiotic effects of LAB on human immune cells. Here, we demonstrated that LAB double-stranded RNA (dsRNA) triggered interferon-β (IFN-β) production by human dendritic cells (DCs), which activated IFN-γ-producing T cells. Interleukin-12 (IL-12) secretion from human DCs in response to LAB was abrogated by depletion of bacterial dsRNA, and was attenuated by neutralizing IFN-β, indicating LAB dsRNA primarily activated the IFN-β/IL-12 pathway. Moreover, the induction of IL-12 secretion from DCs by LAB was abolished by the inhibition of endosomal acidification, confirming the critical role of the endosomal digestion of LAB. In a coculture of human naïve CD4+ T cells and BDCA1+ DCs, DCs stimulated with LAB containing dsRNA induced IFN-γ-producing T cells. These results indicate that human DCs activated by LAB enhance Th1 immunity depending on IFN-β secretion in response to bacterial dsRNA.
|
| Volume |
9
|
| Pages |
27
|
| Published |
2018-1-23
|
| DOI |
10.3389/fimmu.2018.00027
|
| PMID |
29410667
|
| PMC |
PMC5787129
|
| MeSH |
Antigens, CD1 / metabolism
Cells, Cultured
Dendritic Cells / immunology*
Glycoproteins / metabolism
Humans
Interferon-beta / biosynthesis
Interferon-beta / immunology*
Interferon-gamma / biosynthesis
Interferon-gamma / immunology
Interleukin-12 Subunit p35 / metabolism
Lactobacillales / genetics
Lactobacillales / immunology*
RNA, Bacterial / immunology
RNA, Double-Stranded / immunology*
Th1 Cells / immunology*
Th2 Cells / metabolism
|
| IF |
4.716
|
| Times Cited |
5
|
| Resource |
| General Microbes |
JCM 1149
JCM 1558
JCM 5805
JCM 1002
JCM 1136
JCM 5824
JCM 5826
JCM 5827
JCM 9498 |
