論文 - 詳細
| RRC ID | 55195 |
|---|---|
| 著者 | Lee JM, Kim JH, Kim KW, Lee BJ, Kim DG, Kim YO, Lee JH, Kong IS. |
| タイトル | Physicochemical properties, production, and biological functionality of poly-γ-d-glutamic acid with constant molecular weight from halotolerant Bacillus sp. SJ-10. |
| ジャーナル | Int J Biol Macromol |
| Abstract |
Poly-γ-glutamic acid (γ-PGA) is an unusual anionic homopolyamide that is biodegradable, edible, and nontoxic. It has a wide variety of industrial applications depending on its combined cations, and molecular weight. In this study, extracellular viscous γ-PGA produced by halotolerant Bacillus sp. SJ-10 isolated from a traditional Korean salted-fermented seafood was purified and characterized. The physicochemical analysis indicated that the γ-PGA produced by Bacillus sp. SJ-10 consists primarily of d-glutamic acid residues combined with sodium cations. When batch fermentation was performed with 8% NaCl for 3 d, Bacillus sp. SJ-10 produced approximately 24.7g/L γ-PGA with a molecular weight of approximately 400 kilodaltons (kDa). Under fermentation conditions with 6% NaCl, the maximum yield was 26.2g/L regardless of the molecular weight. The γ-PGA sodium salt with a molecular weight of 400kDa exhibited antioxidant activity by scavenging 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radicals and reducing molybdenum, with maximal scavenging activity at 0.5mg/mL and reducing activity at 1mg/mL (20μg ascorbic acid-equivalent), respectively. These results suggest the potential use of γ-PGA in the food, cosmetic, and biomedical industries for its antioxidant qualities. Our results also provide an economical method for controlling the molecular weight of the γ-PGA produced. |
| 巻・号 | 108 |
| ページ | 598-607 |
| 公開日 | 2018-3-1 |
| DOI | 10.1016/j.ijbiomac.2017.12.055 |
| PII | S0141-8130(17)30677-3 |
| PMID | 29229248 |
| MeSH | Amino Acids / chemistry Bacillus / chemistry* Bacillus / metabolism* Fermentation Glutamic Acid Hydrogen-Ion Concentration Magnetic Resonance Spectroscopy Molecular Weight* Phenols / chemistry Phenols / metabolism Plant Extracts / chemistry Plant Extracts / metabolism Polyglutamic Acid / analogs & derivatives* Polyglutamic Acid / chemistry Polyglutamic Acid / metabolism Sodium Chloride Spectroscopy, Fourier Transform Infrared Temperature Thermogravimetry |
| IF | 4.784 |
| 引用数 | 6 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 15790 |