論文 - 詳細
| RRC ID | 55211 |
|---|---|
| 著者 | Sakamoto M, Iino T, Hamada M, Ohkuma M. |
| タイトル | Parolsenella catena gen. nov., sp. nov., isolated from human faeces. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
Strain 2CBEGH3T, which is an obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-stain-positive coccobacillus, was isolated from a faecal sample of a healthy Japanese man. The 16S rRNA gene sequence analysis showed that strain 2CBEGH3T represented a member of the family Atopobiaceae and formed a monophyletic cluster with Olsenella uli DSM 7084T (93.6 % sequence similarity), Olsenella umbonata strain lac31T (93.0 %), Olsenella profusa JCM 14553T (92.7 %) and Olsenella scatoligenes strain SK9K4T (92.7 %) as closest neighbours and Atopobium species. The hsp60 gene sequence analysis supported the phylogenetic relationships based on the 16S rRNA gene sequences, with sequence similarity values of 82.1-84.7 % to the four species described above. A unique three-base (one amino acid residue) insertion was found in the alignment regions of the hsp60 gene sequence of strain 2CBEGH3T. The major end products from d-glucose were d- and l-lactic acids produced at the ratio of 75 : 25, while four species of the genus Olsenella produced d- and l-lactic acids at ratios of 94-98 : 2-6. The isolate formed characteristic crater-like colonies on Eggerth-Gagnon agar plates. The major cellular fatty acids were C18 : 1ω9c, C18 : 1ω9c dimethyl acetal (DMA) and C16 : 0 DMA. The G+C content of the genomic DNA was 68.4 mol%. On the basis of these data, strain 2CBEGH3T represents a novel species in a novel genus of the family Atopobiaceae, for which the name Parolsenella catena gen. nov., sp. nov. is proposed. The type strain of P. catena is 2CBEGH3T (=JCM 31932T=DSM 105194T). |
| 巻・号 | 68(4) |
| ページ | 1165-1172 |
| 公開日 | 2018-4-1 |
| DOI | 10.1099/ijsem.0.002645 |
| PMID | 29458507 |
| MeSH | Actinobacteria / classification* Actinobacteria / genetics Actinobacteria / isolation & purification Adult Bacteria, Anaerobic / classification Bacteria, Anaerobic / genetics Bacteria, Anaerobic / isolation & purification Bacterial Typing Techniques DNA, Bacterial / genetics Fatty Acids / chemistry Feces / microbiology* Genes, Bacterial Humans Japan Male Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA |
| IF | 2.166 |
| 引用数 | 3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 31932 JCM 12494 JCM 14553 JCM 16156 JCM 19907 |