論文 - 詳細
| RRC ID | 55277 |
|---|---|
| 著者 | Kim SH, Kim JG, Jung MY, Kim SJ, Gwak JH, Yu WJ, Roh SW, Kim YH, Rhee SK. |
| タイトル | Ketobacter alkanivorans gen. nov., sp. nov., an n-alkane-degrading bacterium isolated from seawater. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
Strain GI5T was isolated from a surface seawater sample collected from Garorim Bay (West Sea, Republic of Korea). The isolated strain was aerobic, Gram-stain-negative, rod-shaped, motile by means of a polar flagellum, negative for catalase and weakly positive for oxidase. The optimum growth pH, salinity and temperature were determined to be pH 7.5-8.0, 3 % NaCl (w/v) and 25 °C, respectively; the growth ranges were pH 6.0-9.0, 1-7 % NaCl (w/v) and 18-40 °C. The results of phylogenetic analysis of 16S rRNA gene sequences indicated that GI5T clustered within the family Alcanivoracaceae, and most closely with Alcanivorax dieseloleiB-5T and Alcanivorax marinusR8-12T (91.9 % and 91.6 % similarity, respectively). The major cellular fatty acids in GI5T were C18 : 1ω7c/C18 : 1ω6c (44.45 %), C16 : 1ω6c/C16 : 1ω7c (14.17 %) and C16 : 0 (10.19 %); this profile was distinct from those of the closely related species. The major respiratory quinone of GI5T was Q-8. The main polar lipids were phosphatidylethanolamine and phosphatidylglycerol. Two putative alkane hydroxylase (alkB) genes were identified in GI5T. The G+C content of the genomic DNA of GI5T was determined to be 51.2 mol%. On the basis of the results of phenotypic, chemotaxonomic and phylogenetic studies, strain GI5T represents a novel species of a novel genus of the family Alcanivoracaceae, for which we propose the name Ketobacter alkanivorans gen. nov., sp. nov.; the type strain is GI5T (=KCTC 52659T=JCM 31835T). |
| 巻・号 | 68(7) |
| ページ | 2258-2264 |
| 公開日 | 2018-7-1 |
| DOI | 10.1099/ijsem.0.002823 |
| PMID | 29809120 |
| MeSH | Alcanivoraceae / classification* Alcanivoraceae / genetics Alcanivoraceae / isolation & purification Alkanes / metabolism* Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Fatty Acids / chemistry Phospholipids / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Republic of Korea Seawater / microbiology* Sequence Analysis, DNA Ubiquinone / chemistry |
| IF | 2.166 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 31835 |