| Abstract |
A novel endophytic actinobacterium strain, designated A249T, was isolated from the stem of Populus adenopoda collected at Mount Qingcheng in south-west China. Its taxonomic position was determined by using a polyphasic approach. The cultural and morphological characteristics of isolate A249T were consistent with members of the genus Streptomyces. Growth occurred at 10-37 °C, pH 6.0-12.0 and in the presence of 0-4 % (w/v) NaCl. Analysis of the 16S rRNA gene sequence and phylogenetic trees showed the closest phylogenetic relatives to strain A249T were Streptomyces shaanxiensis JCM 16925T (98.0 % 16S rRNA gene sequence similarity) and Streptomyces lanatus JCM 4332T (97.9 %). The DNA-DNA hybridization values between the strain A249T and the two reference strains ranged from 41.4 to 49.4 %. The DNA G+C content was 71.7 mol%. The range of average nucleotide identity values was 81.5-86.7 %. Chemical analysis of cellular components indicated that strain A249T contained ll-diaminopimelic acid, xylose and galactose. The predominant menaquinones were MK-9(H6) and MK-9(H8). The polar lipids were diphosphatidylglycerol, phosphatidylinositol mannosides, phosphatidylethanolamine, two unidentified lipids, one unidentified phospholipid, one unidentified aminolipid and one unidentified aminophospholipid. The major fatty acids comprised C16 : 0, iso-C14 : 0, iso-C15 : 0, iso-C16 : 0, anteiso-C15 : 0 and C16 : 1ω7c. On the basis of the phenotypic and genotypic differentiation of the three tested strains, isolate A249T is proposed to represent a novel species of the genus Streptomyces, named Streptomyces populi sp. nov. The type strain is A249T (=CGMCC 4.7417T=JCM 32175T).
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