論文 - 詳細
| RRC ID | 55297 |
|---|---|
| 著者 | Ramaprasad EVV, Mahidhara G, Sasikala C, Ramana CV. |
| タイトル | Rhodococcus electrodiphilus sp. nov., a marine electro active actinobacterium isolated from coral reef. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
An electrogenic bacterium was isolated from a marine coral, designated as strain JC435T and its taxonomic status examined by using a polyphasic approach. Results from the 16S rRNA gene sequence study showed that the isolate belonged to the genus Rhodococcus and formed a cluster with Rhodococcus ruber KCTC 9806T (99.5 % 16S rRNA gene sequence similarity) and Rhodococcus aetherivorans JCM 14343T (99.3 %), respectively. Genome relatedness based on DNA-DNA hybridization to the type strains of closest-related species was less than 30 % and the ΔTm of >7 °C, suggesting that strain represents a new species of the genus Rhodococcus. The major fatty acids were C16 : 0, C18 : 1ω9c, C18 : 010-methyl and C16 : 1ω6c and/or C16 : 1ω7c. The polar lipids of strain JC435T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside, phosphatidylinositol, three unknown phospholipids and an unknown amino lipid. The major isoprenoid quinone was MK-8(H2), with 8 % of MK-7(H2) and 2 % of MK-9(H2) as minor components. Whole-cell hydrolysates contained meso-diaminopimelic acid, arabinose and galactose as the diagnostic diamino acid and sugars. Mycolic acids were detected. The genomic DNA G+C content of strain JC435T was 69.8 mol%. On the basis of phylogenetic genotypic, physiological and chemotaxonomic analysis, strain JC435T is considered to represent a novel species of the genus Rhodococcus for which the name Rhodococcuselectrodiphilus sp. nov. is proposed. The type strain is JC435T (=KCTC 39856T=LMG 29881T=MCC 3659T). |
| 巻・号 | 68(8) |
| ページ | 2644-2649 |
| 公開日 | 2018-8-1 |
| DOI | 10.1099/ijsem.0.002895 |
| PMID | 29957174 |
| MeSH | Bacterial Typing Techniques Base Composition Coral Reefs* DNA, Bacterial / genetics Diaminopimelic Acid / chemistry Fatty Acids / chemistry India Mycolic Acids / chemistry Nucleic Acid Hybridization Phospholipids / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Rhodococcus / classification* Rhodococcus / genetics Rhodococcus / isolation & purification Sequence Analysis, DNA Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry |
| IF | 2.166 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 一般微生物 | JCM 14343 |