RRC ID 55335
著者 Sujarit K, Kudo T, Ohkuma M, Pathom-Aree W, Lumyong S.
タイトル Streptomyces venetus sp. nov., an actinomycete with a blue aerial mycelium.
ジャーナル Int J Syst Evol Microbiol
Abstract A Gram-positive bacterium, designated CMU-AB225T, was isolated from rhizosphere soil of an oil palm (Elaeis guineensis). The strain exhibited a blue aerial spore mass and a light cream to moderate yellow substrate mycelium and formed chains of spiny spores. Whole-cell hydrolysates consisted of ll-diaminopimelic acid, glucose, ribose, mannose and galactose. The predominant menaquinones were MK-9(H6), MK-9(H8) and MK-9(H4). The polar lipids profile contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol-mannoside, four unidentified lipids, two unidentified aminolipids and an unidentified glycolipid. The major cellular fatty acids (>10 %) were iso-C16 : 0, C16 : 0, anteiso-C15 : 0 and iso-C15 : 0. The G+C content of genomic DNA was 69.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CMU-AB225T was a member of the genus Streptomyces and formed a distinct phyletic line which was most closely related to Streptomyces koyangensis JCM 14915T, Streptomyces misionensis JCM 4497T and Streptomyces aurantiogriseus JCM 4346T. Multilocus sequence analysis (MLSA) using five housekeeping genes (atpD, gyrB, recA, rpoB and trpB) showed that the MLSA distances of strain CMU-AB225T to phylogenetically related species were greater than the 0.007 threshold. Moreover, the low values of DNA-DNA relatedness and phenotypic differences, especially a blue aerial mycelium, enabled strain CMU-AB225T to be distinguished from its closely related species. It is thus proposed that strain CMU-AB225T represents a novel species, namely Streptomyces venetus sp. nov. The type strain is CMU-AB225T (=JCM 31290T=TBRC 2001T).
巻・号 68(10)
ページ 3333-3339
公開日 2018-10-1
DOI 10.1099/ijsem.0.002995
PMID 30168790
MeSH Arecaceae / microbiology* Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Diaminopimelic Acid / chemistry Fatty Acids / chemistry Genes, Bacterial Glycolipids / chemistry Phospholipids / chemistry Phylogeny* Pigmentation RNA, Ribosomal, 16S / genetics Rhizosphere* Sequence Analysis, DNA Soil Microbiology* Streptomyces / classification* Streptomyces / genetics Streptomyces / isolation & purification Thailand Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry
IF 2.166
引用数 0
リソース情報
一般微生物 JCM 31290 JCM 14915 JCM 4497 JCM 4346