RRC ID |
55346
|
著者 |
Tanizawa Y, Tada I, Kobayashi H, Endo A, Maeno S, Toyoda A, Arita M, Nakamura Y, Sakamoto M, Ohkuma M, Tohno M.
|
タイトル |
Lactobacillus paragasseri sp. nov., a sister taxon of Lactobacillus gasseri, based on whole-genome sequence analyses.
|
ジャーナル |
Int J Syst Evol Microbiol
|
Abstract |
Three strains, JCM 5343T, JCM 5344 and JCM 1130, currently identified as Lactobacillus gasseri, were investigated using a polyphasic taxonomic approach. Although these strains shared high 16S rRNA gene sequence similarities with L. gasseri ATCC 33323T (99.9 %), they formed a clade clearly distinct from ATCC 33323T based on whole-genome relatedness. The average nucleotide identity and in silico DNA-DNA hybridization values of these three strains compared to L. gasseri ATCC 33323T were 93.4-93.7 and 53.1-54.1 %, respectively, and both were less than the widely accepted threshold to distinguish two species (95 and 70 %, respectively). The three strains were Gram-stain positive, non-motile, non-spore-forming, catalase-negative and rod-shaped bacteria. They grew at 25-45 °C and in the presence of 2.0 % (w/v) NaCl. The major fatty acids of the three strains were C16 : 0 and C18 : 1 ω9c. Based on phylogenetic analyses of the phenylalanyl-tRNA synthase alpha subunit and RNA polymerase alpha subunit genes, and on phenotypic and chemotaxonomic characteristics, strains JCM 5343T, JCM 5344 and JCM 1130 represent a novel species distinct from L. gasseri, for which we propose the name Lactobacillusparagasseri sp. nov. In addition, a large portion of genomes currently labelled as L. gasseri in the public sequence database should be reclassified as L. paragasseri based on whole-genome relatedness.
|
巻・号 |
68(11)
|
ページ |
3512-3517
|
公開日 |
2018-11-1
|
DOI |
10.1099/ijsem.0.003020
|
PMID |
30226464
|
MeSH |
Bacterial Typing Techniques
DNA, Bacterial / genetics
DNA-Directed RNA Polymerases / genetics
Fatty Acids / chemistry
Genome, Bacterial*
Lactobacillus / classification*
Lactobacillus / genetics
Lactobacillus gasseri
Nucleic Acid Hybridization
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
IF |
2.166
|
引用数 |
4
|
リソース情報 |
一般微生物 |
JCM 5343
JCM 5344
JCM 1130 |