RRC ID 55346
著者 Tanizawa Y, Tada I, Kobayashi H, Endo A, Maeno S, Toyoda A, Arita M, Nakamura Y, Sakamoto M, Ohkuma M, Tohno M.
タイトル Lactobacillus paragasseri sp. nov., a sister taxon of Lactobacillus gasseri, based on whole-genome sequence analyses.
ジャーナル Int J Syst Evol Microbiol
Abstract Three strains, JCM 5343T, JCM 5344 and JCM 1130, currently identified as Lactobacillus gasseri, were investigated using a polyphasic taxonomic approach. Although these strains shared high 16S rRNA gene sequence similarities with L. gasseri ATCC 33323T (99.9 %), they formed a clade clearly distinct from ATCC 33323T based on whole-genome relatedness. The average nucleotide identity and in silico DNA-DNA hybridization values of these three strains compared to L. gasseri ATCC 33323T were 93.4-93.7 and 53.1-54.1 %, respectively, and both were less than the widely accepted threshold to distinguish two species (95 and 70 %, respectively). The three strains were Gram-stain positive, non-motile, non-spore-forming, catalase-negative and rod-shaped bacteria. They grew at 25-45 °C and in the presence of 2.0 % (w/v) NaCl. The major fatty acids of the three strains were C16 : 0 and C18 : 1 ω9c. Based on phylogenetic analyses of the phenylalanyl-tRNA synthase alpha subunit and RNA polymerase alpha subunit genes, and on phenotypic and chemotaxonomic characteristics, strains JCM 5343T, JCM 5344 and JCM 1130 represent a novel species distinct from L. gasseri, for which we propose the name Lactobacillusparagasseri sp. nov. In addition, a large portion of genomes currently labelled as L. gasseri in the public sequence database should be reclassified as L. paragasseri based on whole-genome relatedness.
巻・号 68(11)
ページ 3512-3517
公開日 2018-11-1
DOI 10.1099/ijsem.0.003020
PMID 30226464
MeSH Bacterial Typing Techniques DNA, Bacterial / genetics DNA-Directed RNA Polymerases / genetics Fatty Acids / chemistry Genome, Bacterial* Lactobacillus / classification* Lactobacillus / genetics Lactobacillus gasseri Nucleic Acid Hybridization Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA
IF 2.166
引用数 4
リソース情報
一般微生物 JCM 5343 JCM 5344 JCM 1130